PDBsum entry 3u4q

Hydrolase/DNA

PDB id: 3u4q

Contents

Protein chains

1122 a.a.

1132 a.a.

DNA/RNA

Ligands

SO4

EDO

Waters ×189

PDB id:

3u4q

Name:

Hydrolase/DNA

Title:

Structure of addab-DNA complex at 2.8 angstroms

Structure:

Atp-dependent helicase/nuclease subunit a. Chain: a. Synonym: atp-dependent helicase/nuclease adda. Engineered: yes. Mutation: yes. Atp-dependent helicase/deoxyribonuclease subunit b. Chain: b. Synonym: atp-dependent helicase/nuclease addb. Engineered: yes.

Source:

Bacillus subtilis. Organism_taxid: 1423. Gene: adda, bsu10630. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: addb, bsu10620. Synthetic: yes

Resolution:

2.80Å R-factor: 0.236 R-free: 0.287

Authors:

K.Saikrishnan,W.Krajewski,D.Wigley

Key ref:

K.Saikrishnan et al. (2012). Insights into Chi recognition from the structure of an AddAB-type helicase-nuclease complex. Embo J, 31, 1568-1578. PubMed id: 22307084

Date:

10-Oct-11 Release date: 21-Mar-12

Protein chain

P23478  (ADDA_BACSU) -  ATP-dependent helicase/nuclease subunit A from Bacillus subtilis (strain 168)

Seq: 1232 a.a.

Struc: 1122 a.a.*

Protein chain

P23477  (ADDB_BACSU) -  ATP-dependent helicase/deoxyribonuclease subunit B from Bacillus subtilis (strain 168)

Seq: 1166 a.a.

Struc: 1132 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

DNA/RNA chain

T-A-A-T-G-C-G-A-G-C-G-C-T-C-G-C-A-T-T-A-G-A-T-T-T-T-G 27 bases

Enzyme reactions

• Enzyme class 2: Chains A, B: E.C.3.1.-.-
• Enzyme class 3: Chain A: E.C.5.6.2.4 - Dna 3'-5' helicase.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Embo J 31:1568-1578 (2012)

PubMed id: 22307084

Insights into Chi recognition from the structure of an AddAB-type helicase-nuclease complex.

K.Saikrishnan, J.T.Yeeles, N.S.Gilhooly, W.W.Krajewski, M.S.Dillingham, D.B.Wigley.

ABSTRACT

No abstract given.