PDBsum entry 3to0

Oxidoreductase

PDB id

3to0

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Contents

			Protein chains

					184 a.a.

			Ligands

					FMN ×2


					PO4 ×2


					ACT ×2

			Waters ×50

PDB id:

3to0

Links

Name:

Oxidoreductase

Title:

Crystal structure of mus musculus iodotyrosine deiodinase (iyd) c217a, c239a bound to fmn

Structure:

Iodotyrosine deiodinase 1. Chain: a, b. Synonym: iyd-1. Engineered: yes. Mutation: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Strain: 10090. Gene: iyd-1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.66Å

R-factor:

0.185

R-free:

0.237

Authors:

J.M.Buss,P.M.Mctamney,S.E.Rokita

Key ref:

J.M.Buss et al. (2012). Expression of a soluble form of iodotyrosine deiodinase for active site characterization by engineering the native membrane protein from Mus musculus. Protein Sci, 21, 351-361. PubMed id: 22238141

Date:

02-Sep-11

Release date:

29-Feb-12

PROCHECK

	Headers

	References

Protein chains

Q9DCX8 (IYD1_MOUSE) - Iodotyrosine deiodinase 1 from Mus musculus

Seq: Struc:					285 a.a. 184 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 2 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.1.21.1.1 - iodotyrosine deiodinase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

2 iodide + L-tyrosine + 2 NADP⁺ = 3,5-diiodo-L-tyrosine + 2 NADPH + H⁺

2 × iodide	+	L-tyrosine	+	2 × NADP(+)	=	3,5-diiodo-L-tyrosine	+	2 × NADPH	+	H(+)

Cofactor:

FMN

FMN
Bound ligand (Het Group name = FMN) corresponds exactly

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	Protein Sci 21:351-361 (2012)
PubMed id: 22238141

Expression of a soluble form of iodotyrosine deiodinase for active site characterization by engineering the native membrane protein from Mus musculus.
J.M.Buss, P.M.McTamney, S.E.Rokita.

ABSTRACT

No abstract given.