PDBsum entry 3syt

Ligase

PDB id: 3syt

Contents

Protein chains

660 a.a.

Ligands

GLU ×4

NAD ×4

AMP ×4

POP ×4

GOL ×2

Waters ×486

PDB id:

3syt

Name:

Ligase

Title:

Crystal structure of glutamine-dependent NAD+ synthetase from m. Tuberculosis bound to amp/ppi, NAD+, and glutamate

Structure:

Glutamine-dependent NAD(+) synthetase. Chain: a, b, c, d. Synonym: NAD(+) synthase [glutamine-hydrolyzing]. Engineered: yes

Source:

Mycobacterium tuberculosis. Organism_taxid: 1773. Gene: mt2513, mtcy428.08, nade, rv2438c. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.65Å R-factor: 0.157 R-free: 0.200

Authors:

W.Chuenchor,B.Gerratana

Key ref:

W.Chuenchor et al. (2012). Regulation of the intersubunit ammonia tunnel in Mycobacterium tuberculosis glutamine-dependent NAD+ synthetase. Biochem J, 443, 417-426. PubMed id: 22280445

Date:

18-Jul-11 Release date: 11-Apr-12

Protein chains

P9WJJ3  (NADE_MYCTU) -  Glutamine-dependent NAD(+) synthetase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 679 a.a.

Struc: 660 a.a.

Enzyme reactions

• Enzyme class: E.C.6.3.5.1 - NAD(+) synthase (glutamine-hydrolyzing).
• Reaction: deamido-NAD⁺ + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD⁺ + H⁺

deamido-NAD(+) + L-glutamine + ATP + H2O = L-glutamate + AMP (Bound ligand (Het Group name = POP) corresponds exactly) + diphosphate + NAD(+) (Bound ligand (Het Group name = GLU) corresponds exactly) + H(+) (Bound ligand (Het Group name = NAD) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biochem J 443:417-426 (2012)

PubMed id: 22280445

Regulation of the intersubunit ammonia tunnel in Mycobacterium tuberculosis glutamine-dependent NAD+ synthetase.

W.Chuenchor, T.I.Doukov, M.Resto, A.Chang, B.Gerratana.

ABSTRACT

No abstract given.