PDBsum entry 3sw6

Lyase

PDB id: 3sw6

Contents

Protein chain

214 a.a.

Ligands

5FU

Waters ×127

PDB id:

3sw6

Name:

Lyase

Title:

Crystal structure of methanothermobacter thermautotrophicus orotidine 5'-monophosphate decarboxylase covalently modified by 5-fluoro-6- azido-ump

Structure:

Orotidine 5'-phosphate decarboxylase. Chain: a. Synonym: omp decarboxylase, ompdcase, ompdecase. Engineered: yes

Source:

Methanothermobacter thermautotrophicus. Methanococcus thermolithotrophicus. Organism_taxid: 187420. Strain: deltah. Gene: 1470090, mth_129, pyrf. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.50Å R-factor: 0.162 R-free: 0.189

Authors:

Y.Liu,L.P.Kotra,E.F.Pai

Key ref:

Y.Liu et al. Crystal structure of methanothermobacter thermautotro orotidine 5'-Monophosphate decarboxylase covalently m by 5-Fluoro-6-Azido-Ump. To be published, .

Date:

13-Jul-11 Release date: 02-May-12

Protein chain

O26232  (PYRF_METTH) -  Orotidine 5'-phosphate decarboxylase from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)

Seq: 228 a.a.

Struc: 214 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.1.1.23 - orotidine-5'-phosphate decarboxylase.
• Pathway: Pyrimidine Biosynthesis
• Reaction: orotidine 5'-phosphate + H⁺ = UMP + CO2

orotidine 5'-phosphate + H(+) = UMP + CO2 (Bound ligand (Het Group name = 5FU) matches with 95.45% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site