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PDBsum entry 3s8c
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Oxidoreductase
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PDB id
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3s8c
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PDB id:
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| Name: |
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Oxidoreductase
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Title:
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Structure of yeast ribonucleotide reductase 1 r293a with amppnp and cdp
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Structure:
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Ribonucleoside-diphosphate reductase large chain 1. Chain: a. Fragment: ribonucleotide reductase 1. Synonym: ribonucleotide reductase r1 subunit 1, ribonucleotide reductase large subunit 1. Engineered: yes. Mutation: yes
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Source:
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Saccharomyces cerevisiae s288c. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: crt7, rir1, rnr1, saccharomyces, sds12, yer070w. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.77Å
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R-factor:
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0.202
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R-free:
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0.256
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Authors:
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M.F.Ahmad,P.S.Kaushal,Q.Wan,S.R.Wijeratna,M.Huang,C.D.Dealwis
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Key ref:
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M.F.Ahmad
et al.
Structural and biochemical basis of lethal mutant r29 yeast ribonucleotide reductase.
To be published,
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Date:
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27-May-11
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Release date:
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11-Apr-12
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PROCHECK
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Headers
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References
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P21524
(RIR1_YEAST) -
Ribonucleoside-diphosphate reductase large chain 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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Seq:
Struc:
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888 a.a.
666 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class:
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E.C.1.17.4.1
- ribonucleoside-diphosphate reductase.
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Reaction:
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a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol
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2'-deoxyribonucleoside diphosphate
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+
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thioredoxin disulfide
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+
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H(2)O
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=
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ribonucleoside diphosphate
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+
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thioredoxin
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Cofactor:
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Fe(3+) or adenosylcob(III)alamin or Mn(2+)
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Fe(3+)
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or
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adenosylcob(III)alamin
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or
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Mn(2+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
