PDBsum entry 3s5o

Lyase

PDB id: 3s5o

Contents

Protein chain

296 a.a.

Ligands

EDO ×6

Metals

__K

Waters ×211

PDB id:

3s5o

Name:

Lyase

Title:

Crystal structure of human 4-hydroxy-2-oxoglutarate aldolase bound to pyruvate

Structure:

4-hydroxy-2-oxoglutarate aldolase, mitochondrial. Chain: a. Synonym: dihydrodipicolinate synthase-like, dhdps-like protein, 2- keto-4-hydroxyglutarate aldolase, khg-aldolase, protein 569272. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: c10orf65, dhdpsl, hoga1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.97Å R-factor: 0.197 R-free: 0.216

Authors:

T.J.Riedel,W.T.Lowther

Key ref:

T.J.Riedel et al. (2011). Structural and biochemical studies of human 4-hydroxy-2-oxoglutarate aldolase: implications for hydroxyproline metabolism in primary hyperoxaluria. Plos One, 6, e26021. PubMed id: 21998747

Date:

23-May-11 Release date: 26-Oct-11

Protein chain

Q86XE5  (HOGA1_HUMAN) -  4-hydroxy-2-oxoglutarate aldolase, mitochondrial from Homo sapiens

Seq: 327 a.a.

Struc: 296 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.1.3.16 - 4-hydroxy-2-oxoglutarate aldolase.
• Reaction:
  1. (4R)-4-hydroxy-2-oxoglutarate = glyoxylate + pyruvate
  2. (4S)-4-hydroxy-2-oxoglutarate = glyoxylate + pyruvate

(4R)-4-hydroxy-2-oxoglutarate = glyoxylate (Bound ligand (Het Group name = EDO) matches with 80.00% similarity) + pyruvate

(4S)-4-hydroxy-2-oxoglutarate = glyoxylate (Bound ligand (Het Group name = EDO) matches with 80.00% similarity) + pyruvate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Plos One 6:e26021 (2011)

PubMed id: 21998747

Structural and biochemical studies of human 4-hydroxy-2-oxoglutarate aldolase: implications for hydroxyproline metabolism in primary hyperoxaluria.

T.J.Riedel, L.C.Johnson, J.Knight, R.R.Hantgan, R.P.Holmes, W.T.Lowther.

ABSTRACT

No abstract given.