PDBsum entry 3r9a

Transferase/transport protein

PDB id: 3r9a

Contents

Protein chains

387 a.a.

289 a.a.

Ligands

BTB

Waters ×831

PDB id:

3r9a

Name:

Transferase/transport protein

Title:

Human alanine-glyoxylate aminotransferase in complex with the tpr domain of human pex5p

Structure:

Serine--pyruvate aminotransferase. Chain: a, c. Synonym: spt, alanine--glyoxylate aminotransferase, agt. Engineered: yes. Peroxisomal targeting signal 1 receptor. Chain: b, d. Fragment: tpr-domain, unp residues 315-639. Synonym: pts1 receptor, pts1r, pts1-bp, peroxin-5, peroxisomal c- terminal targeting signal import receptor, peroxisome receptor 1.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: agt1, agxt, spat. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: pex5, pxr1.

Resolution:

2.35Å R-factor: 0.187 R-free: 0.239

Authors:

K.Fodor,M.Wilmanns

Key ref:

K.Fodor et al. (2012). Molecular requirements for peroxisomal targeting of alanine-glyoxylate aminotransferase as an essential determinant in primary hyperoxaluria type 1. Plos Biol, 10, e1001309. PubMed id: 22529745

Date:

25-Mar-11 Release date: 11-May-11

Supersedes:

3imz

Protein chains

P21549  (SPYA_HUMAN) -  Alanine--glyoxylate aminotransferase from Homo sapiens

Seq: 392 a.a.

Struc: 387 a.a.*

Protein chains

P50542  (PEX5_HUMAN) -  Peroxisomal targeting signal 1 receptor from Homo sapiens

Seq: 639 a.a.

Struc: 289 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chains A, C: E.C.2.6.1.44 - alanine--glyoxylate transaminase.
• Reaction: glyoxylate + L-alanine = glycine + pyruvate
• Cofactor: Pyridoxal 5'-phosphate
• Enzyme class 3: Chains A, C: E.C.2.6.1.51 - serine--pyruvate transaminase.
• Reaction: L-serine + pyruvate = 3-hydroxypyruvate + L-alanine

L-serine (Bound ligand (Het Group name = BTB) matches with 40.00% similarity) + pyruvate = 3-hydroxypyruvate + L-alanine

• Cofactor: Pyridoxal 5'-phosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Plos Biol 10:e1001309 (2012)

PubMed id: 22529745

Molecular requirements for peroxisomal targeting of alanine-glyoxylate aminotransferase as an essential determinant in primary hyperoxaluria type 1.

K.Fodor, J.Wolf, R.Erdmann, W.Schliebs, M.Wilmanns.

ABSTRACT

No abstract given.