PDBsum entry 3r0l

Toxin/hydrolase

PDB id: 3r0l

Contents

Protein chains

33 a.a.

32 a.a.

122 a.a.

Ligands

ASN-CYS-GLN

GLN-PRO-CYS

ACT

Metals

_CL

_MN

_NA ×2

Waters ×265

PDB id:

3r0l

Name:

Toxin/hydrolase

Title:

Crystal structure of crotoxin

Structure:

Crotoxin chain a. Chain: a. Fragment: crotoxin acidic subunit isoform ca2 alpha chain. Synonym: ca, crotapotin, crotoxin chain a. Crotoxin chain b. Chain: b. Fragment: crotoxin acidic subunit isoform ca2 beta chain. Synonym: ca, crotapotin, crotoxin chain b. Crotoxin chain c.

Source:

Crotalus durissus terrificus. South american rattlesnake,cascabel. Organism_taxid: 8732. Other_details: from south american rattlesnake venom. Other_details: from south american rattlesnake venom

Resolution:

1.35Å R-factor: 0.166 R-free: 0.189

Authors:

F.A.Saul,G.Faure

Key ref:

G.Faure et al. (2011). Crystal structure of crotoxin reveals key residues involved in the stability and toxicity of this potent heterodimeric β-neurotoxin. J Mol Biol, 412, 176-191. PubMed id: 21787789

Date:

08-Mar-11 Release date: 12-Oct-11

Protein chain

P08878  (PA2H_CRODU) -  Phospholipase A2 homolog crotoxin acid subunit CA from Crotalus durissus terrificus

Seq: 138 a.a.

Struc: 33 a.a.

Protein chain

P08878  (PA2H_CRODU) -  Phospholipase A2 homolog crotoxin acid subunit CA from Crotalus durissus terrificus

Seq: 138 a.a.

Struc: 32 a.a.

Protein chain

P0CG56  (PA2BB_CRODU) -  Phospholipase A2 crotoxin basic subunit CBb from Crotalus durissus terrificus

Seq: 122 a.a.

Struc: 122 a.a.

Enzyme reactions

• Enzyme class: Chain D: E.C.3.1.1.4 - phospholipase A2.
• Reaction: a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3- phosphocholine + a fatty acid + H⁺

1,2-diacyl-sn-glycero-3-phosphocholine + H2O = 1-acyl-sn-glycero-3- phosphocholine + fatty acid (Bound ligand (Het Group name = ACT) matches with 60.00% similarity) + H(+)

• Cofactor: Ca(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Mol Biol 412:176-191 (2011)

PubMed id: 21787789

Crystal structure of crotoxin reveals key residues involved in the stability and toxicity of this potent heterodimeric β-neurotoxin.

G.Faure, H.Xu, F.A.Saul.

ABSTRACT

No abstract given.