PDBsum entry 3ql2

Hydrolase

PDB id: 3ql2

Contents

Protein chains

124 a.a.

Ligands

SO4 ×2

Waters ×249

PDB id:

3ql2

Name:

Hydrolase

Title:

Crystal structure of ribonuclease a variant a4c/d83e/v118c

Structure:

Ribonuclease pancreatic. Chain: a, b. Synonym: rnase 1, rnase a. Engineered: yes. Mutation: yes

Source:

Bos taurus. Bovine. Organism_taxid: 9913. Gene: rnase1, rns1. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.49Å R-factor: 0.169 R-free: 0.209

Authors:

U.Arnold,M.Schoepfel

Key ref:

U.Arnold and M.Schöpfel (2012). Significant stabilization of ribonuclease A by additive effects. Febs J, 279, 2508-2519. PubMed id: 22594773

Date:

02-Feb-11 Release date: 29-Feb-12

Protein chains

P61823  (RNAS1_BOVIN) -  Ribonuclease pancreatic from Bos taurus

Seq: 150 a.a.

Struc: 124 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.4.6.1.18 - pancreatic ribonuclease.
• Reaction:
  1. an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]
  2. an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]

Febs J 279:2508-2519 (2012)

PubMed id: 22594773

Significant stabilization of ribonuclease A by additive effects.

U.Arnold, M.Schöpfel.

ABSTRACT

No abstract given.