PDBsum entry 3q7u

Transferase

PDB id: 3q7u

Contents

Protein chains

222 a.a.

Ligands

CTP ×2

Metals

_MG ×2

Waters ×396

PDB id:

3q7u

Name:

Transferase

Title:

Structure of mtb 2-c-methyl-d-erythritol 4-phosphate cytidyltransferase (ispd) complexed with ctp

Structure:

2-c-methyl-d-erythritol 4-phosphate cytidyltransferase. Chain: a, b. Synonym: 4-diphosphocytidyl-2c-methyl-d-erythritol synthase, mep cytidylyltransferase, mct. Engineered: yes

Source:

Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: h37rv. Gene: ispd, rv3582c, mt3688, mtcy06g11.29c. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.10Å R-factor: 0.191 R-free: 0.236

Authors:

M.C.M.Reddy,J.B.Bruning,C.Thurman,T.R.Ioerger,J.C.Sacchettini,Tb Structural Genomics Consortium (Tbsgc)

Key ref:

M.C.M.Reddy et al. Crystal structure of mycobacterium tuberculosis 2-C-Methyl-D-Erythritol 4-Phosphate cytidyltransferas (ispd): a candidate antitubercular drug target. Proteins, .

Date:

05-Jan-11 Release date: 04-May-11

Protein chains

P9WKG9  (ISPD_MYCTU) -  2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 231 a.a.

Struc: 222 a.a.

Enzyme reactions

• Enzyme class: E.C.2.7.7.60 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase.
• Reaction: 2-C-methyl-D-erythritol 4-phosphate + CTP + H⁺ = 4-CDP-2-C-methyl-D- erythritol + diphosphate

2-C-methyl-D-erythritol 4-phosphate + CTP (Bound ligand (Het Group name = CTP) corresponds exactly) + H(+) = 4-CDP-2-C-methyl-D- erythritol + diphosphate

• Cofactor: Mn(2+) or Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site