PDBsum entry 3py8

Transferase/DNA

PDB id: 3py8

Contents

Protein chain

539 a.a.

DNA/RNA

Ligands

DCT

GOL ×2

Metals

_MN ×5

_MG

Waters ×523

PDB id:

3py8

Name:

Transferase/DNA

Title:

Crystal structure of a mutant of the large fragment of DNA polymerase i from thermus aquaticus in a closed ternary complex with DNA and ddctp

Structure:

DNA polymerase i. Chain: a. Fragment: DNA polymerase i large fragment. Synonym: taq polymerase 1. Engineered: yes. Mutation: yes. DNA (5'-d( Gp Ap Cp Cp Ap Cp Gp Gp Cp Gp Cp (Doc))-3'). Chain: b. Engineered: yes.

Source:

Thermus aquaticus. Organism_taxid: 271. Gene: pola, pol1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes

Resolution:

1.74Å R-factor: 0.171 R-free: 0.199

Authors:

A.Schnur,A.Marx,W.Welte,K.Diederichs

Key ref:

S.Obeid et al. (2011). Learning from directed evolution: Thermus aquaticus DNA polymerase mutants with translesion synthesis activity. Chembiochem, 12, 1574-1580. PubMed id: 21480455

Date:

12-Dec-10 Release date: 15-Jun-11

Protein chain

P19821  (DPO1_THEAQ) -  DNA polymerase I, thermostable from Thermus aquaticus

Seq: 832 a.a.

Struc: 539 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

G-A-C-C-A-C-G-G-C-G-C-DOC 12 bases

A-G-G-G-C-G-C-C-G-T-G-G-T-C 14 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Chembiochem 12:1574-1580 (2011)

PubMed id: 21480455

Learning from directed evolution: Thermus aquaticus DNA polymerase mutants with translesion synthesis activity.

S.Obeid, A.Schnur, C.Gloeckner, N.Blatter, W.Welte, K.Diederichs, A.Marx.

ABSTRACT

No abstract given.