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PDBsum entry 3pco
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protein ligands Protein-protein interface(s) links
Ligase PDB id
3pco

 

 

 

 

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Contents
Protein chains
242 a.a. *
795 a.a. *
323 a.a. *
Ligands
PHE ×2
AMP ×2
* Residue conservation analysis
PDB id:
3pco
Name: Ligase
Title: Crystal structure of e. Coli phenylalanine-tRNA synthetase complexed with phenylalanine and amp
Structure: Phenylalanyl-tRNA synthetase, alpha subunit. Chain: a, c. Fragment: ligase. Engineered: yes. Phenylalanyl-tRNA synthetase, beta chain. Chain: b, d. Engineered: yes
Source: Escherichia coli. Organism_taxid: 562. Gene: ecdh1_1928. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
3.02Å     R-factor:   0.235     R-free:   0.300
Authors: I.Mermershtain,I.Finarov,L.Klipcan,N.Kessler,H.Rozenberg,M.G.Safro
Key ref: I.Mermershtain et al. (2011). Idiosyncrasy and identity in the prokaryotic Phe-system: crystal structure of E. coli phenylalanyl-tRNA synthetase complexed with phenylalanine and AMP. Protein Sci, 20, 160-167. PubMed id: 21082706
Date:
21-Oct-10     Release date:   02-Mar-11    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P08312  (SYFA_ECOLI) -  Phenylalanine--tRNA ligase alpha subunit from Escherichia coli (strain K12)
Seq:
Struc: 		327 a.a.
242 a.a.
Protein chains
Pfam   ArchSchema ?
P07395  (SYFB_ECOLI) -  Phenylalanine--tRNA ligase beta subunit from Escherichia coli (strain K12)
Seq:
Struc: 		 
Seq:
Struc: 		795 a.a.
795 a.a.
Protein chain
Pfam   ArchSchema ?
P08312  (SYFA_ECOLI) -  Phenylalanine--tRNA ligase alpha subunit from Escherichia coli (strain K12)
Seq:
Struc: 		327 a.a.
323 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chains A, B, C, D: E.C.6.1.1.20  - phenylalanine--tRNA ligase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H+
tRNA(Phe)
 +
L-phenylalanine
Bound ligand (Het Group name = PHE)
corresponds exactly 		+ ATP
 =
L-phenylalanyl-tRNA(Phe)
Bound ligand (Het Group name = AMP)
corresponds exactly 		+ AMP
 + diphosphate
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Protein Sci 20:160-167 (2011)
PubMed id: 21082706  
 
 
Idiosyncrasy and identity in the prokaryotic Phe-system: crystal structure of E. coli phenylalanyl-tRNA synthetase complexed with phenylalanine and AMP.
I.Mermershtain, I.Finarov, L.Klipcan, N.Kessler, H.Rozenberg, M.G.Safro.
 
  ABSTRACT  
 
No abstract given.

 

 

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