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PDBsum entry 3p4r
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Oxidoreductase
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PDB id
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3p4r
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Contents |
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577 a.a.
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243 a.a.
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130 a.a.
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119 a.a.
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* Residue conservation analysis
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PDB id:
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| Name: |
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Oxidoreductase
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Title:
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Crystal structure of menaquinol:fumarate oxidoreductase in complex with glutarate
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Structure:
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Fumarate reductase flavoprotein subunit. Chain: a, m. Fragment: unp residues 1-577. Engineered: yes. Fumarate reductase iron-sulfur subunit. Chain: b, n. Engineered: yes. Fumarate reductase subunit c. Chain: c, o.
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Source:
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Escherichia coli 042. Organism_taxid: 216592. Strain: 042/eaec. Gene: ec042_4630, frda, sdy_4398. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: frdb, ec042_4629. Escherichia coli o55:h7. Organism_taxid: 701177.
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Resolution:
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3.05Å
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R-factor:
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0.249
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R-free:
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0.284
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Authors:
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T.M.Tomasiak,T.L.Archuleta,J.Andrell,C.Luna-Chavez,T.A.Davis, M.Sarwar,A.J.Ham,W.H.Mcdonald,V.Yankowskaya,H.A.Stern,J.N.Johnston, E.Maklashina,G.Cecchini,T.M.Iverson
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Key ref:
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T.M.Tomasiak
et al.
(2011).
Geometric restraint drives on- and off-pathway catalysis by the Escherichia coli menaquinol:fumarate reductase.
J Biol Chem,
286,
3047-3056.
PubMed id:
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Date:
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07-Oct-10
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Release date:
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24-Nov-10
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PROCHECK
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Headers
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References
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P00363
(FRDA_ECOLI) -
Fumarate reductase flavoprotein subunit from Escherichia coli (strain K12)
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Seq:
Struc:
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602 a.a.
577 a.a.
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P0AC47
(FRDB_ECOLI) -
Fumarate reductase iron-sulfur subunit from Escherichia coli (strain K12)
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Seq:
Struc:
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244 a.a.
243 a.a.
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Enzyme class:
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Chains A, B, M, N:
E.C.1.3.5.1
- succinate dehydrogenase.
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Pathway:
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Citric acid cycle
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Reaction:
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a quinone + succinate = fumarate + a quinol
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quinone
Bound ligand (Het Group name = )
matches with 54.55% similarity
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+
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succinate
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=
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fumarate
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+
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quinol
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Cofactor:
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FAD; Iron-sulfur
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FAD
Bound ligand (Het Group name =
FAD)
corresponds exactly
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Iron-sulfur
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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J Biol Chem
286:3047-3056
(2011)
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PubMed id:
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Geometric restraint drives on- and off-pathway catalysis by the Escherichia coli menaquinol:fumarate reductase.
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T.M.Tomasiak,
T.L.Archuleta,
J.Andréll,
C.Luna-Chávez,
T.A.Davis,
M.Sarwar,
A.J.Ham,
W.H.McDonald,
V.Yankovskaya,
H.A.Stern,
J.N.Johnston,
E.Maklashina,
G.Cecchini,
T.M.Iverson.
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ABSTRACT
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