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PDBsum entry 3og3
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De novo protein, lyase
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PDB id
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3og3
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PDB id:
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| Name: |
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De novo protein, lyase
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Title:
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Crystal structure of an artificial thermostable (ba)8-barrel protein from identical half barrels
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Structure:
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Imidazole glycerol phosphate synthase subunit hisf. Chain: a. Synonym: igp synthase cyclase subunit, igp synthase subunit hisf, imgp synthase subunit hisf, igps subunit hisf. Engineered: yes. Mutation: yes
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Source:
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Thermotoga maritima. Organism_taxid: 2336. Gene: hisf, hisf derivative (amino acids 99-219), tm_1036. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.08Å
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R-factor:
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0.152
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R-free:
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0.196
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Authors:
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J.M.Sperl,M.Bocola,F.List,B.Kellerer,R.Sterner
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Key ref:
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J.M.Sperl
et al.
Design of an artificial thermostable (ba)8-Barrel pro from identical half barrels.
To be published,
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Date:
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16-Aug-10
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Release date:
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17-Aug-11
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PROCHECK
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Headers
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References
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Q9X0C6
(HIS6_THEMA) -
Imidazole glycerol phosphate synthase subunit HisF from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
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Seq:
Struc:
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253 a.a.
242 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 98 residue positions (black
crosses)
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Enzyme class:
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E.C.4.3.2.10
- imidazole glycerol-phosphate synthase.
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Reaction:
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5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta- D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol- 4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D- ribosyl)imidazole-4-carboxamide + L-glutamate + H+
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5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta- D-ribosyl)imidazole-4-carboxamide
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+
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L-glutamine
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=
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D-erythro-1-(imidazol- 4-yl)glycerol 3-phosphate
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+
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5-amino-1-(5-phospho-beta-D- ribosyl)imidazole-4-carboxamide
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+
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L-glutamate
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+
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H(+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
