PDBsum entry 3nvc

Oxidoreductase

PDB id: 3nvc

Contents

Protein chain

349 a.a.

Ligands

SAL

GOL ×3

Metals

FE2

Waters ×173

PDB id:

3nvc

Name:

Oxidoreductase

Title:

Crystal structure of salicylate 1,2-dioxygenase g106a mutant from pseudoaminobacter salicylatoxidans in complex with salicylate

Structure:

Gentisate 1,2-dioxygenase. Chain: a. Engineered: yes. Mutation: yes

Source:

Pseudaminobacter salicylatoxidans. Organism_taxid: 93369. Strain: bn12. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.45Å R-factor: 0.197 R-free: 0.272

Authors:

M.Ferraroni,F.Briganti,I.Matera

Key ref:

M.Ferraroni et al. (2013). The salicylate 1,2-dioxygenase as a model for a conventional gentisate 1,2-dioxygenase: crystal structures of the G106A mutant and its adducts with gentisate and salicylate. Febs J, 280, 1643-1652. PubMed id: 23384287 DOI: 10.1111/febs.12173

Date:

08-Jul-10 Release date: 13-Jul-11

Protein chain

Q67FT0  (Q67FT0_PSESE) -  Gentisate 1,2-dioxygenase from Pseudaminobacter salicylatoxidans

Seq: 368 a.a.

Struc: 349 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.13.11.4 - gentisate 1,2-dioxygenase.
• Reaction: 2,5-dihydroxybenzoate + O2 = 3-maleylpyruvate + H⁺

2,5-dihydroxybenzoate (Bound ligand (Het Group name = SAL) matches with 90.91% similarity) + O2 = 3-maleylpyruvate + H(+)

• Cofactor: Fe cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1111/febs.12173

Febs J 280:1643-1652 (2013)

PubMed id: 23384287

The salicylate 1,2-dioxygenase as a model for a conventional gentisate 1,2-dioxygenase: crystal structures of the G106A mutant and its adducts with gentisate and salicylate.

M.Ferraroni, I.Matera, S.Bürger, S.Reichert, L.Steimer, A.Scozzafava, A.Stolz, F.Briganti.

ABSTRACT

No abstract given.