PDBsum entry 3n6r

Ligase

PDB id

3n6r

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Contents

			Protein chains

					591 a.a. *


					(+ 0 more) 506 a.a. *


					646 a.a. *

			Ligands

					BTI ×6

* Residue conservation analysis

PDB id:

3n6r

Links

Name:

Ligase

Title:

Crystal structure of the holoenzyme of propionyl-coa carboxylase (pcc)

Structure:

Propionyl-coa carboxylase, alpha subunit. Chain: a, c, e, g, i, k. Engineered: yes. Propionyl-coa carboxylase, beta subunit. Chain: b, d, f, h, j, l. Engineered: yes

Source:

Ruegeria pomeroyi. Organism_taxid: 89184. Gene: pcca, spo1101. Expressed in: escherichia coli. Expression_system_taxid: 562. Roseobacter denitrificans. Organism_taxid: 375451. Strain: och 114. Gene: pccb, rd1_2028.

Resolution:

3.20Å

R-factor:

0.212

R-free:

0.245

Authors:

C.S.Huang,K.Sadre-Bazzaz,L.Tong

Key ref:

C.S.Huang et al. (2010). Crystal structure of the alpha(6)beta(6) holoenzyme of propionyl-coenzyme A carboxylase. Nature, 466, 1001-1005. PubMed id: 20725044

Date:

26-May-10

Release date:

25-Aug-10

PROCHECK

	Headers

	References

Protein chains

Q5LUF3 (Q5LUF3_RUEPO) - Propionyl-CoA carboxylase alpha chain from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

Seq: Struc:

Seq: Struc:					681 a.a. 591 a.a.

Protein chains

Q168G2 (Q168G2_ROSDO) - Propionyl-CoA carboxylase beta chain from Roseobacter denitrificans (strain ATCC 33942 / OCh 114)

Seq: Struc:					510 a.a. 506 a.a.

Protein chains

Q5LUF3 (Q5LUF3_RUEPO) - Propionyl-CoA carboxylase alpha chain from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

Seq: Struc:

Seq: Struc:					681 a.a. 646 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

Chains A, B, C, D, E, F, G, H, I, J, K, L: E.C.6.4.1.3 - propionyl-CoA carboxylase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl-CoA + ADP + phosphate + H⁺

propanoyl-CoA	+	hydrogencarbonate	+	ATP	=	(S)-methylmalonyl-CoA	+	ADP	+	phosphate	+	H(+)

Cofactor:

Biotin

Biotin
Bound ligand (Het Group name = BTI) matches with 93.75% similarity

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	Nature 466:1001-1005 (2010)
PubMed id: 20725044

Crystal structure of the alpha(6)beta(6) holoenzyme of propionyl-coenzyme A carboxylase.
C.S.Huang, K.Sadre-Bazzaz, Y.Shen, B.Deng, Z.H.Zhou, L.Tong.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

22158123

C.S.Huang, P.Ge, Z.H.Zhou, and L.Tong (2012).
An unanticipated architecture of the 750-kDa α6β6 holoenzyme of 3-methylcrotonyl-CoA carboxylase.

Nature, 481, 219-223.

PDB codes:

3u9r 3u9s 3u9t

21338175

S.A.Claridge, J.J.Schwartz, and P.S.Weiss (2011).
Electrons, photons, and force: quantitative single-molecule measurements from physics to biology.

ACS Nano, 5, 693-729.

21135213

L.P.Yu, Y.S.Kim, and L.Tong (2010).
Mechanism for the inhibition of the carboxyltransferase domain of acetyl-coenzyme A carboxylase by pinoxaden.

Proc Natl Acad Sci U S A, 107, 22072-22077.

PDB code:

3pgq

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.