PDBsum entry 3mml

Hydrolase

PDB id: 3mml

Contents

Protein chains

289 a.a. *

210 a.a. *

Metals

_CL

Waters ×885

* Residue conservation analysis

PDB id:

3mml

Name:

Hydrolase

Title:

Allophanate hydrolase complex from mycobacterium smegmatis, msmeg0435- msmeg0436

Structure:

Allophanate hydrolase subunit 2. Chain: a, c, e, g. Engineered: yes. Allophanate hydrolase subunit 1. Chain: b, d, f, h. Engineered: yes

Source:

Mycobacterium smegmatis. Organism_taxid: 246196. Strain: mc2 155. Gene: msmeg0435, msmeg_0435. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: msmeg0436, msmeg_0436.

Resolution:

2.50Å R-factor: 0.170 R-free: 0.202

Authors:

M.Kaufmann,I.Chernishof,A.Shin,D.Germano,M.R.Sawaya,G.S.Waldo, M.A.Arbing,J.Perry,D.Eisenberg,Integrated Center For Structure And Function Innovation (Isfi),Tb Structural Genomics Consortium (Tbsgc)

Key ref:

M.Kaufmann et al. Crystal structure of allphanate hydrolase complex fro smegmatis, Msmeg0435-Msmeg0436. To be published, .

Date:

20-Apr-10 Release date: 28-Apr-10

Protein chains

A0QPL0  (A0QPL0_MYCS2) -  Allophanate hydrolase subunit 2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

Seq: 294 a.a.

Struc: 289 a.a.

Protein chains

A0QPL1  (A0QPL1_MYCS2) -  Allophanate hydrolase subunit 1 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

Seq: 210 a.a.

Struc: 210 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, C, E, G: E.C.3.5.1.54 - allophanate hydrolase.
• Reaction: urea-1-carboxylate + H2O + 3 H⁺ = 2 NH4⁺ + 2 CO2

Molecule diagrams generated from .mol files obtained from the KEGG ftp site