PDBsum entry 3l1y

Ligase

PDB id: 3l1y

Contents

Protein chain

152 a.a. *

Waters ×176

* Residue conservation analysis

PDB id:

3l1y

Name:

Ligase

Title:

Crystal structure of human ubc4 e2 conjugating enzyme

Structure:

Ubiquitin-conjugating enzyme e2 d2. Chain: a. Fragment: residues 1-147. Synonym: ubiquitin-protein ligase d2, ubiquitin carrier protein d2, ubiquitin-conjugating enzyme e2-17 kda 2, e2(17)kb 2. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ube2d2, ubc4, ubch5b. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.60Å R-factor: 0.189 R-free: 0.229

Authors:

R.Benirschke,J.R.Thompson,G.Mer

Key ref:

R.C.Benirschke et al. (2010). Molecular basis for the association of human E4B U box ubiquitin ligase with E2-conjugating enzymes UbcH5c and Ubc4. Structure, 18, 955-965. PubMed id: 20696396

Date:

14-Dec-09 Release date: 05-May-10

Protein chain

P62837  (UB2D2_HUMAN) -  Ubiquitin-conjugating enzyme E2 D2 from Homo sapiens

Seq: 147 a.a.

Struc: 152 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: E.C.2.3.2.23 - E2 ubiquitin-conjugating enzyme.
• Reaction: S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine
• Enzyme class 3: E.C.2.3.2.24 - (E3-independent) E2 ubiquitin-conjugating enzyme.
• Reaction: S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N₆- monoubiquitinyl-[acceptor protein]-L-lysine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Structure 18:955-965 (2010)

PubMed id: 20696396

Molecular basis for the association of human E4B U box ubiquitin ligase with E2-conjugating enzymes UbcH5c and Ubc4.

R.C.Benirschke, J.R.Thompson, Y.Nominé, E.Wasielewski, N.Juranić, S.Macura, S.Hatakeyama, K.I.Nakayama, M.V.Botuyan, G.Mer.

ABSTRACT

No abstract given.