PDBsum entry 3jsp

Hydrolase/DNA

PDB id: 3jsp

Contents

Protein chains

194 a.a. *

DNA/RNA

Waters ×11

* Residue conservation analysis

PDB id:

3jsp

Name:

Hydrolase/DNA

Title:

Classic protein with a new twist: crystal structure of a lexa repressor DNA complex

Structure:

Lexa repressor. Chain: a, b. Engineered: yes. Mutation: yes. DNA (5'- d( Tp Ap Tp Ap Cp Tp Gp Tp Ap Tp Gp Cp Gp Cp Ap Tp Ap Cp Ap Gp Tp A)- 3'). Chain: c, d. Engineered: yes

Source:

Escherichia coli k-12. Organism_taxid: 83333. Strain: k12. Gene: lexa, exra, spr, tsl, umua, b4043, jw4003. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes

Resolution:

2.90Å R-factor: 0.267 R-free: 0.287

Authors:

A.P.P.Zhang,Y.Z.Pigli,P.A.Rice

Key ref:

A.P.Zhang et al. (2010). Structure of the LexA-DNA complex and implications for SOS box measurement. Nature, 466, 883-886. PubMed id: 20703307

Date:

10-Sep-09 Release date: 18-Aug-10

Protein chains

P0A7C2  (LEXA_ECOLI) -  LexA repressor from Escherichia coli (strain K12)

Seq: 202 a.a.

Struc: 194 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

T-A-T-A-C-T-G-T-A-T-G-C-G-C-A-T-A-C-A-G-T-A 22 bases

T-A-T-A-C-T-G-T-A-T-G-C-G-C-A-T-A-C-A-G-T-A 22 bases

Enzyme reactions

• Enzyme class: E.C.3.4.21.88 - repressor LexA.
• Reaction: Hydrolysis of Ala-|-Gly bond in repressor lexA.

Nature 466:883-886 (2010)

PubMed id: 20703307

Structure of the LexA-DNA complex and implications for SOS box measurement.

A.P.Zhang, Y.Z.Pigli, P.A.Rice.

ABSTRACT

No abstract given.