PDBsum entry 3js5

Hydrolase

PDB id: 3js5

Contents

Protein chain

156 a.a. *

Ligands

EPE

Metals

_NA

Waters ×164

* Residue conservation analysis

PDB id:

3js5

Name:

Hydrolase

Title:

Crystal structure of protein tyrosine phosphatase from entamoeba histolytica with hepes in the active site. High resolution, alternative crystal form with 1 molecule in asymmetric unit

Structure:

Protein tyrosine phosphatase. Chain: a. Engineered: yes

Source:

Entamoeba histolytica. Organism_taxid: 294381. Strain: hm-1:imss. Gene: ehi_153650. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.94Å R-factor: 0.169 R-free: 0.212

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

A.S.Linford et al. (2014). Crystal structure and putative substrate identification for the Entamoeba histolytica low molecular weight tyrosine phosphatase. Mol Biochem Parasitol, 193, 33-44. PubMed id: 24548880 DOI: 10.1016/j.molbiopara.2014.01.003

Date:

09-Sep-09 Release date: 22-Sep-09

Protein chain

C4LSE7  (C4LSE7_ENTHI) -  acid phosphatase from Entamoeba histolytica (strain ATCC 30459 / HM-1:IMSS / ABRM)

Seq: 157 a.a.

Struc: 156 a.a.

Enzyme reactions

• Enzyme class: E.C.3.1.3.2 - acid phosphatase.
• Reaction: a phosphate monoester + H2O = an alcohol + phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.molbiopara.2014.01.003

Mol Biochem Parasitol 193:33-44 (2014)

PubMed id: 24548880

Crystal structure and putative substrate identification for the Entamoeba histolytica low molecular weight tyrosine phosphatase.

A.S.Linford, N.M.Jiang, T.E.Edwards, N.E.Sherman, W.C.Van Voorhis, L.J.Stewart, P.J.Myler, B.L.Staker, W.A.Petri.

ABSTRACT

No abstract given.