PDBsum entry 3imb

Hydrolase/DNA

PDB id: 3imb

Contents

Protein chains

238 a.a. *

DNA/RNA

Waters ×729

* Residue conservation analysis

PDB id:

3imb

Name:

Hydrolase/DNA

Title:

Alternative binding mode of restriction endonuclease bcni to cognate DNA

Structure:

R.Bcni. Chain: a, b, c, d. Engineered: yes. 5'-d( Gp Tp Cp Cp Gp Gp Gp Cp G)-3'. Chain: e, g, i, k. Engineered: yes. Other_details: g strand. 5'-d( Cp Gp Cp Cp Cp Gp Gp Ap C)-3'. Chain: f, h, j, l.

Source:

Brevibacillus centrosporus. Organism_taxid: 54910. Gene: bcnir. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes

Resolution:

1.89Å R-factor: 0.214 R-free: 0.251

Authors:

M.Sokolowska,M.Kaus-Drobek,H.Czapinska,G.Tamulaitis, R.H.Szczepanowski,V.Siksnys,M.Bochtler

Key ref:

M.Sokolowska et al. How bcni and mvai distinguish w from s. To be published, .

Date:

10-Aug-09 Release date: 11-Aug-10

Protein chains

Q8RNV8  (Q8RNV8_9BACL) -  R.BcnI from Brevibacillus centrosporus

Seq: 238 a.a.

Struc: 238 a.a.

DNA/RNA chains

G-T-C-C-G-G-G-C-G 9 bases

C-G-C-C-C-G-G-A-C 9 bases

G-T-C-C-G-G-G-C-G 9 bases

C-G-C-C-C-G-G-A-C 9 bases

G-T-C-C-G-G-G-C-G 9 bases

C-G-C-C-C-G-G-A-C 9 bases

G-T-C-C-G-G-G-C-G 9 bases

C-G-C-C-C-G-G-A-C 9 bases