PDBsum entry 3g6m

Hydrolase/hydrolase inhibitor

PDB id

3g6m

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Contents

			Protein chain

					388 a.a. *

			Ligands

					CFF ×2

			Waters ×662

* Residue conservation analysis

PDB id:

3g6m

Links

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of a chitinase crchi1 from the nematophagous fungus clonostachys rosea in complex with a potent inhibitor caffeine

Structure:

Chitinase. Chain: a. Fragment: residues 21-426. Synonym: crchi1. Engineered: yes

Source:

Bionectria ochroleuca. Gliocladium roseum. Organism_taxid: 29856. Gene: crchi1. Expressed in: escherichia coli. Expression_system_taxid: 469008. Other_details: synonym is clonostachys rosea

Resolution:

1.65Å

R-factor:

0.185

R-free:

0.230

Authors:

Z.Gan,J.Yang,Z.Lou,Z.Rao,K.-Q.Zhang

Key ref:

J.Yang et al. (2010). Crystal structure and mutagenesis analysis of chitinase CrChi1 from the nematophagous fungus Clonostachys rosea in complex with the inhibitor caffeine. Microbiology, 156, 3566-3574. PubMed id: 20829286

Date:

06-Feb-09

Release date:

16-Feb-10

PROCHECK

	Headers

	References

Protein chain

A9LI60 (A9LI60_BIOOC) - chitinase from Bionectria ochroleuca

Seq: Struc:					426 a.a. 388 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.3.2.1.14 - chitinase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

Hydrolysis of the 1,4-beta-linkages of N-acetyl-D-glucosamine polymers of chitin.

	Microbiology 156:3566-3574 (2010)
PubMed id: 20829286

Crystal structure and mutagenesis analysis of chitinase CrChi1 from the nematophagous fungus Clonostachys rosea in complex with the inhibitor caffeine.
J.Yang, Z.Gan, Z.Lou, N.Tao, Q.Mi, L.Liang, Y.Sun, Y.Guo, X.Huang, C.Zou, Z.Rao, Z.Meng, K.Q.Zhang.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21110018

J.Yang, X.Zhao, L.Liang, Z.Xia, L.Lei, X.Niu, C.Zou, and K.Q.Zhang (2011).
Overexpression of a cuticle-degrading protease Ver112 increases the nematicidal activity of Paecilomyces lilacinus.

Appl Microbiol Biotechnol, 89, 1895-1903.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.