PDBsum entry 3e99

Oxidoreductase

PDB id: 3e99

Contents

Protein chain

149 a.a. *

Ligands

ACT

Waters ×142

* Residue conservation analysis

PDB id:

3e99

Name:

Oxidoreductase

Title:

Crystal structure of the beta subunit of the benzoate 1,2-dioxygenase (benb, bmaa0186) from burkholderia mallei atcc 23344 at 1.90 a resolution

Structure:

Benzoate 1,2-dioxygenase beta subunit. Chain: a. Engineered: yes

Source:

Burkholderia mallei atcc 23344. Pseudomonas mallei. Organism_taxid: 243160. Gene: yp_105014.1, benb, bmaa0186. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.90Å R-factor: 0.188 R-free: 0.223

Authors:

Joint Center For Structural Genomics (Jcsg)

Key ref:

Joint center for structural genomics (jcsg) Crystal structure of the beta subunit of benzoate 1,2-Dioxygenase (yp_105014.1) from burkholderia mallei atcc 23344 at 1.90 a resolution. To be published, .

Date:

21-Aug-08 Release date: 16-Sep-08

Protein chain

A0A0H2WBM4  (A0A0H2WBM4_BURMA) -  Benzoate 1,2-dioxygenase beta subunit from Burkholderia mallei (strain ATCC 23344)

Seq: 163 a.a.

Struc: 149 a.a.

Enzyme reactions

• Enzyme class: E.C.1.14.12.10 - benzoate 1,2-dioxygenase.
• Pathway: Benzoate Metabolism
• Reaction: benzoate + NADH + O2 + H⁺ = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1- carboxylate + NAD⁺

benzoate (Bound ligand (Het Group name = ACT) matches with 44.44% similarity) + NADH + O2 + H(+) = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1- carboxylate + NAD(+)

• Cofactor: Fe(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site