PDBsum entry 3e95

Ligase

PDB id: 3e95

Contents

Protein chains

147 a.a. *

138 a.a. *

Ligands

UNX-UNX-UNX-UNX-UNX

Waters ×130

* Residue conservation analysis

PDB id:

3e95

Name:

Ligase

Title:

Crystal structure of the plasmodium falciparum ubiquitin conjugating enzyme complex, pfubc13-pfuev1a

Structure:

Ubiquitin carrier protein. Chain: a, b. Engineered: yes. Ubiquitin-conjugating enzyme e2. Chain: c. Engineered: yes

Source:

Plasmodium falciparum 3d7. Organism_taxid: 36329. Gene: pfe1350c. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: mal3p2.20, pfc0255c.

Resolution:

2.50Å R-factor: 0.249 R-free: 0.258

Authors:

A.K.Wernimont,A.Lam,A.Ali,S.Brokx,Y.H.Lin,Y.Zhao,J.Lew, M.Ravichandran,G.Wasney,M.Vedadi,I.Kozieradzki,M.Schapira, A.Bochkarev,M.Wilkstrom,C.Bountra,C.H.Arrowsmith,A.M.Edwards,R.Hui, W.Qiu,V.B.Brand,Structural Genomics Consortium (Sgc)

Key ref:

A.K.Wernimont et al. Crystal structure of the plasmodium falciparum ubiquitin conjugating enzyme complex, Pfubc13-Pfuev1a. To be published, .

Date:

21-Aug-08 Release date: 30-Sep-08

Protein chains

Q8I3J4  (Q8I3J4_PLAF7) -  Ubiquitin-conjugating enzyme E2 N, putative from Plasmodium falciparum (isolate 3D7)

Seq: 152 a.a.

Struc: 147 a.a.

Protein chain

O97241  (O97241_PLAF7) -  Ubiquitin-conjugating enzyme E2, putative from Plasmodium falciparum (isolate 3D7)

Seq: 142 a.a.

Struc: 138 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C: E.C.6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45.
• Reaction: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine

Molecule diagrams generated from .mol files obtained from the KEGG ftp site