PDBsum entry 3do2

Hydrolase

PDB id: 3do2

Contents

Protein chain

316 a.a. *

Ligands

VAL-LYS

Metals

_CA ×4

_ZN

Waters ×185

* Residue conservation analysis

PDB id:

3do2

Name:

Hydrolase

Title:

Thermolysin by lb nanotemplate method after high x-ray dose on esrf id14-2 beamline

Structure:

Thermolysin. Chain: a. Synonym: thermostable neutral proteinase. Ec: 3.4.24.27

Source:

Bacillus thermoproteolyticus. Organism_taxid: 1427

Resolution:

1.22Å R-factor: 0.239 R-free: 0.261

Authors:

E.Pechkova,S.K.Tripathi,C.Nicolini

Key ref:

E.Pechkova et al. Radiation damage in protein structural characterization by synchrotron radiation: state of the art and nanotechnology-Based perspective. To be published, .

Date:

03-Jul-08 Release date: 07-Jul-09

Protein chain

P00800  (THER_BACTH) -  Thermolysin from Bacillus thermoproteolyticus

Seq: 548 a.a.

Struc: 316 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.24.27 - thermolysin.
• Reaction: Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe.
• Cofactor: Ca(2+); Zn(2+)