PDBsum entry 3cy3

Transferase

PDB id: 3cy3

Contents

Protein chain

271 a.a. *

Ligands

ARG-LYS-ARG-ARG-ARG-HIS-PRO-SER

EDO

JN5

Waters ×218

* Residue conservation analysis

PDB id:

3cy3

Name:

Transferase

Title:

Crystal structure of human proto-oncogene serine threonine kinase (pim1) in complex with a consensus peptide and the jnk inhibitor v

Structure:

Serine/threonine-protein kinase pim-1. Chain: a. Engineered: yes. Mutation: yes. Pimtide peptide. Chain: b. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: pim1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630

Resolution:

2.15Å R-factor: 0.165 R-free: 0.205

Authors:

P.Filippakopoulos,A.Bullock,O.Fedorov,A.C.W.Pike,F.Von Delft, C.H.Arrowsmith,A.M.Edwards,C.Bountra,S.Knapp,Structural Genomics Consortium (Sgc)

Key ref:

P.Filippakopoulos et al. Proto-Oncogene serine threonine kinase (pim1) in complex with a consensus peptide and the jnk inhibitor V.. To be published, .

Date:

25-Apr-08 Release date: 15-Jul-08

Protein chain

P11309  (PIM1_HUMAN) -  Serine/threonine-protein kinase pim-1 from Homo sapiens

Seq: 313 a.a.

Struc: 271 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site