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PDBsum entry 3cu8
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Protein binding, signaling protein
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PDB id
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3cu8
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Protein binding, signaling protein
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Title:
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Impaired binding of 14-3-3 to raf1 is linked to noonan and leopard syndrome
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Structure:
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14-3-3 protein zeta/delta. Chain: a, b. Synonym: 14-3-3 zeta adapter protein, protein kinasE C inhibitor protein 1, kcip-1. Engineered: yes. Raf proto-oncogene serine/threonine-protein kinase. Chain: p, q. Fragment: phosphorylated craf1 peptide. Synonym: raf-1, c-raf, craf.
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: ywhaz. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: raf1pser259 peptide (nh2-255qrstpstpnvha265-cooh) was synthesized by biosyntan (berlin, germany).
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Resolution:
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2.40Å
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R-factor:
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0.192
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R-free:
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0.259
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Authors:
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B.Schumacher,M.Weyand,J.Kuhlmann,C.Ottmann
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Key ref:
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M.Molzan
et al.
(2010).
Impaired binding of 14-3-3 to C-RAF in Noonan syndrome suggests new approaches in diseases with increased Ras signaling.
Mol Cell Biol,
30,
4698-4711.
PubMed id:
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Date:
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16-Apr-08
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Release date:
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05-May-09
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PROCHECK
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Headers
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References
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P63104
(1433Z_HUMAN) -
14-3-3 protein zeta/delta from Homo sapiens
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Seq:
Struc:
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245 a.a.
229 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Enzyme class:
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E.C.2.7.11.1
- non-specific serine/threonine protein kinase.
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Reaction:
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1.
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L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H+
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2.
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L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H+
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L-seryl-[protein]
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+
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ATP
Bound ligand (Het Group name = )
matches with 50.00% similarity
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=
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O-phospho-L-seryl-[protein]
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+
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ADP
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+
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H(+)
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L-threonyl-[protein]
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+
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ATP
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=
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O-phospho-L-threonyl-[protein]
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+
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ADP
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+
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H(+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Mol Cell Biol
30:4698-4711
(2010)
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PubMed id:
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Impaired binding of 14-3-3 to C-RAF in Noonan syndrome suggests new approaches in diseases with increased Ras signaling.
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M.Molzan,
B.Schumacher,
C.Ottmann,
A.Baljuls,
L.Polzien,
M.Weyand,
P.Thiel,
R.Rose,
M.Rose,
P.Kuhenne,
M.Kaiser,
U.R.Rapp,
J.Kuhlmann,
C.Ottmann.
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ABSTRACT
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Links
