PDBsum entry 3ax0

Oxidoreductase/metal transport

PDB id: 3ax0

Contents

Protein chains

273 a.a.

78 a.a.

Ligands

NO3 ×5

Metals

_CU ×4

Waters ×338

PDB id:

3ax0

Name:

Oxidoreductase/metal transport

Title:

Crystal structure of streptomyces tyrosinase in a complex with caddie y98f mutant soaked in a cu(ii)-containing solution for 80 hr

Structure:

Tyrosinase. Chain: a. Engineered: yes. Melc. Chain: b. Synonym: caddie protein orf378. Engineered: yes. Mutation: yes

Source:

Streptomyces castaneoglobisporus. Organism_taxid: 79261. Strain: hut 6202. Gene: tyrc. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: orf378.

Resolution:

1.40Å R-factor: 0.170 R-free: 0.195

Authors:

Y.Matoba,M.Sugiyama

Key ref:

Y.Matoba et al. (2011). A molecular mechanism for copper transportation to tyrosinase that is assisted by a metallochaperone, caddie protein. J Biol Chem, 286, 30219-30231. PubMed id: 21730070

Date:

26-Mar-11 Release date: 29-Jun-11

Protein chain

Q83WS2  (Q83WS2_9ACTN) -  Tyrosinase from Streptomyces castaneoglobisporus

Seq: 273 a.a.

Struc: 273 a.a.*

Protein chain

Q83WS1  (Q83WS1_9ACTN) -  MelC from Streptomyces castaneoglobisporus

Seq: 126 a.a.

Struc: 78 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.1.14.18.1 - tyrosinase.
• Pathway: Melanin Biosynthesis
• Reaction:
  1. L-tyrosine + O2 = L-dopaquinone + H2O
  2. 2 L-dopa + O2 = 2 L-dopaquinone + 2 H2O
• Cofactor: Cu cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Biol Chem 286:30219-30231 (2011)

PubMed id: 21730070

A molecular mechanism for copper transportation to tyrosinase that is assisted by a metallochaperone, caddie protein.

Y.Matoba, N.Bando, K.Oda, M.Noda, F.Higashikawa, T.Kumagai, M.Sugiyama.

ABSTRACT

No abstract given.