PDBsum entry 3ai9

Transferase

PDB id: 3ai9

Contents

Protein chain

211 a.a.

Ligands

SAM

Metals

_MG ×2

Waters ×174

PDB id:

3ai9

Name:

Transferase

Title:

Crystal structure of duf358 protein reveals a putative spout-class rrna methyltransferase

Structure:

Upf0217 protein mj1640. Chain: x. Engineered: yes. Mutation: yes

Source:

Methanocaldococcus jannaschii. Methanococcus jannaschii. Organism_taxid: 2190. Gene: mj1640. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.55Å R-factor: 0.192 R-free: 0.237

Authors:

Y.A.Yuan,H.Y.Chen

Key ref:

H.Y.Chen and Y.A.Yuan (2010). Crystal structure of Mj1640/DUF358 protein reveals a putative SPOUT-class RNA methyltransferase. J Mol Cell Biol, 2, 366-374. PubMed id: 21098051 DOI: 10.1093/jmcb/mjq034

Date:

11-May-10 Release date: 30-Mar-11

Protein chain

Q59034  (TRMY_METJA) -  tRNA (pseudouridine(54)-N(1))-methyltransferase from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

Seq: 205 a.a.

Struc: 211 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.1.1.257 - tRNA (pseudouridine(54)-N(1))-methyltransferase.
• Reaction: pseudouridine₅₄ in tRNA + S-adenosyl-L-methionine = N₁- methylpseudouridine₅₄ in tRNA + S-adenosyl-L-homocysteine + H⁺

pseudouridine(54) in tRNA + S-adenosyl-L-methionine = N(1)- methylpseudouridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+) (Bound ligand (Het Group name = SAM) matches with 76.92% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1093/jmcb/mjq034

J Mol Cell Biol 2:366-374 (2010)

PubMed id: 21098051

Crystal structure of Mj1640/DUF358 protein reveals a putative SPOUT-class RNA methyltransferase.

H.Y.Chen, Y.A.Yuan.

ABSTRACT

No abstract given.