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PDBsum entry 3ad8
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protein ligands metals Protein-protein interface(s) links
Oxidoreductase PDB id
3ad8

 

 

 

 

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JSmol PyMol  
Contents
Protein chains
963 a.a. *
404 a.a. *
195 a.a. *
91 a.a. *
Ligands
NAD
SO4 ×12
FAD
FMN
PYC
Metals
_ZN
Waters ×1020
* Residue conservation analysis
PDB id:
3ad8
Name: Oxidoreductase
Title: Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with pyrrole 2-carboxylate
Structure: Sarcosine oxidase alpha subunit. Chain: a. Engineered: yes. Sarcosine oxidase beta subunit. Chain: b. Engineered: yes. Sarcosine oxidase gamma subunit. Chain: c. Fragment: unp residues 11-205.
Source: Corynebacterium sp. U-96. Organism_taxid: 31944. Gene: soxa. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: soxb. Gene: soxg. Gene: soxd.
Resolution:
2.20Å     R-factor:   0.163     R-free:   0.206
Authors: H.Suzuki,T.Moriguchi,K.Ida
Key ref: T.Moriguchi et al. (2010). Channeling and conformational changes in the heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96. J Biochem (tokyo), 148, 491-505. PubMed id: 20675294
Date:
15-Jan-10     Release date:   25-Aug-10    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q50LF0  (Q50LF0_9CORY) -  Sarcosine oxidase subunit alpha from Corynebacterium sp. U-96
Seq:
Struc: 		 
Seq:
Struc: 		965 a.a.
963 a.a.
Protein chain
Pfam   ArchSchema ?
Q50LF2  (Q50LF2_9CORY) -  Subunit beta of sarcosine oxidase from Corynebacterium sp. U-96
Seq:
Struc: 		405 a.a.
404 a.a.
Protein chain
Pfam   ArchSchema ?
Q50LE9  (Q50LE9_9CORY) -  Subunit gamma of sarcosine oxidase from Corynebacterium sp. U-96
Seq:
Struc: 		205 a.a.
195 a.a.
Protein chain
Pfam   ArchSchema ?
Q50LF1  (Q50LF1_9CORY) -  Subunit delta of sarcosine oxidase from Corynebacterium sp. U-96
Seq:
Struc: 		99 a.a.
91 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chains A, B: E.C.1.5.3.1  - sarcosine oxidasee (formaldehyde-forming).
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: sarcosine + O2 + H2O = formaldehyde + glycine + H2O2
sarcosine
 + O2
 + H2O
Bound ligand (Het Group name = PYC)
matches with 75.00% similarity 		= formaldehyde
 + glycine
 + H2O2
      Cofactor: FAD
FAD
Bound ligand (Het Group name = FAD) corresponds exactly
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
J Biochem (tokyo) 148:491-505 (2010)
PubMed id: 20675294  
 
 
Channeling and conformational changes in the heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96.
T.Moriguchi, K.Ida, T.Hikima, G.Ueno, M.Yamamoto, H.Suzuki.
 
  ABSTRACT  
 
No abstract given.

 

 

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