PDBsum entry 3a2n

Hydrolase

PDB id

3a2n

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Contents

			Protein chains

					298 a.a. *

			Metals

					_CL ×3

			Waters ×490

* Residue conservation analysis

PDB id:

3a2n

Links

Name:

Hydrolase

Title:

Crystal structure of dbja (wild type type ii p21)

Structure:

Haloalkane dehalogenase. Chain: a, b, e, f. Engineered: yes

Source:

Bradyrhizobium japonicum. Organism_taxid: 375. Strain: usda110. Gene: dbja. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.89Å

R-factor:

0.193

R-free:

0.228

Authors:

Y.Sato,T.Senda

Key ref:

Z.Prokop et al. (2010). Enantioselectivity of haloalkane dehalogenases and its modulation by surface loop engineering. Angew Chem Int Ed Engl, 49, 6111-6115. PubMed id: 20645368

Date:

23-May-09

Release date:

26-May-10

PROCHECK

	Headers

	References

Protein chains

P59337 (DHAA_BRADU) - Haloalkane dehalogenase from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)

Seq: Struc:					310 a.a. 298 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.3.8.1.5 - haloalkane dehalogenase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1-haloalkane + H2O = a halide anion + a primary alcohol + H⁺

1-haloalkane	+	H2O	=	halide anion	+	primary alcohol	+	H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

	Angew Chem Int Ed Engl 49:6111-6115 (2010)
PubMed id: 20645368

Enantioselectivity of haloalkane dehalogenases and its modulation by surface loop engineering.
Z.Prokop, Y.Sato, J.Brezovsky, T.Mozga, R.Chaloupkova, T.Koudelakova, P.Jerabek, V.Stepankova, R.Natsume, J.G.van Leeuwen, D.B.Janssen, J.Florian, Y.Nagata, T.Senda, J.Damborsky.

ABSTRACT

No abstract given.