PDBsum entry 3a2f

Transferase/replication

PDB id: 3a2f

Contents

Protein chains

773 a.a. *

247 a.a. *

Waters ×126

* Residue conservation analysis

PDB id:

3a2f

Name:

Transferase/replication

Title:

Crystal structure of pyrococcus furiosus DNA polymerase/pcna monomer mutant complex

Structure:

DNA polymerase. Chain: a. Synonym: replicative b-type polymerase, pfu polymerase. Engineered: yes. DNA polymerase sliding clamp. Chain: b. Synonym: DNA clamp, proliferating cell nuclear antigen homolog, pcna. Engineered: yes. Mutation: yes

Source:

Pyrococcus furiosus. Organism_taxid: 2261. Gene: pol, pf0212. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: pcn, pf0983.

Resolution:

2.67Å R-factor: 0.241 R-free: 0.284

Authors:

H.Nishida,Y.Ishino,K.Morikawa

Key ref:

H.Nishida et al. Structural determinant for switching between the polymerase and exonuclease modes in the pcna-Replicative DNA polymerase complex. To be published, . PubMed id: 19934045

Date:

15-May-09 Release date: 03-Nov-09

Protein chain

P61875  (DPOL_PYRFU) -  DNA polymerase from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Seq: 775 a.a.

Struc: 773 a.a.

Protein chain

O73947  (PCNA_PYRFU) -  DNA polymerase sliding clamp from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Seq: 249 a.a.

Struc: 247 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site