PDBsum entry 2zpp

Hormone

PDB id

2zpp

Loading ...

Contents

			Protein chains

					21 a.a.


					30 a.a. *

			Ligands

					DOD ×34

* Residue conservation analysis

PDB id:

2zpp

Links
- PDBe
- RCSB
- MMDB
- JenaLib
- Proteopedia
- CATH
- SCOP
- PDBSWS
- ProSAT

Name:

Hormone

Title:

Neutron crystal structure of cubic insulin at pd9

Structure:

Insulin a chain. Chain: a. Insulin b chain. Chain: b

Source:

Sus scrofa. Pig. Organism_taxid: 9823. Tissue: pancreas. Tissue: pancreas

Authors:

T.Ishikawa,I.Tanaka,N.Niimura

Key ref:

T.Ishikawa et al. (2008). An abnormal pK(a) value of internal histidine of the insulin molecule revealed by neutron crystallographic analysis. Biochem Biophys Res Commun, 376, 32-35. PubMed id: 18725203

Date:

26-Jul-08

Release date:

28-Oct-08

PROCHECK

	Headers

	References

Protein chain

P01315 (INS_PIG) - Insulin from Sus scrofa

Seq: Struc:					108 a.a. 21 a.a.

Protein chain

P01315 (INS_PIG) - Insulin from Sus scrofa

Seq: Struc:					108 a.a. 30 a.a.

Key:

PfamA domain

Secondary structure

	Biochem Biophys Res Commun 376:32-35 (2008)
PubMed id: 18725203

An abnormal pK(a) value of internal histidine of the insulin molecule revealed by neutron crystallographic analysis.
T.Ishikawa, T.Chatake, Y.Morimoto, M.Maeda, K.Kurihara, I.Tanaka, N.Niimura.

ABSTRACT

Insulin is stored in pancreatic beta-cell as hexameric form with Zn2+ ions, while the hormonally active form is monomer. The hexamer requires the coordination of Zn2+ ions to the HisB10. In order to reveal the mechanism of the hexamerization of insulin, we investigated the Zn2+ free insulin at pD6.6 and pD9 by neutron crystallographic analyses. HisB10 is doubly protonated not only at pD6.6 but also at pD9, indicating an abnormal pK(a) of this histidine. It is suggested that HisB10 acts on a strong cation capture and contributes to the high stability of the hexameric form in pancreas.

Literature references that cite this PDB file's key reference

PubMed id

Reference

19770501

W.Iwai, T.Yamada, K.Kurihara, Y.Ohnishi, Y.Kobayashi, I.Tanaka, H.Takahashi, R.Kuroki, T.Tamada, and N.Niimura (2009).
A neutron crystallographic analysis of T6 porcine insulin at 2.1 A resolution.

Acta Crystallogr D Biol Crystallogr, 65, 1042-1050.

PDB code:

3fhp

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.