PDBsum entry 2yeq

Hydrolase

PDB id: 2yeq

Contents

Protein chains

522 a.a.

Ligands

PE5 ×30

EDO ×43

PO4 ×2

Metals

_NA ×6

_FE ×2

_CA ×4

Waters ×625

PDB id:

2yeq

Name:

Hydrolase

Title:

Structure of phod

Structure:

Alkaline phosphatase d. Chain: a, b. Synonym: apased, phod. Engineered: yes

Source:

Bacillus subtilis. Organism_taxid: 1423. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.93Å R-factor: 0.180 R-free: 0.202

Authors:

J.E.D.Lillington,F.Rodriguez,P.Roversi,S.J.Johnson,B.Berks,S.M.Lea

Key ref:

F.Rodriguez et al. (2014). Crystal structure of the Bacillus subtilis phosphodiesterase PhoD reveals an iron and calcium-containing active site. J Biol Chem, 289, 30889-30899. PubMed id: 25217636 DOI: 10.1074/jbc.M114.604892

Date:

30-Mar-11 Release date: 18-Apr-12

Protein chains

P42251  (PPBD_BACSU) -  Alkaline phosphatase D from Bacillus subtilis (strain 168)

Seq: 583 a.a.

Struc: 522 a.a.

Enzyme reactions

• Enzyme class: E.C.3.1.3.1 - alkaline phosphatase.
• Reaction: a phosphate monoester + H2O = an alcohol + phosphate

phosphate monoester + H2O = alcohol + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

• Cofactor: Mg(2+); Zn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1074/jbc.M114.604892

J Biol Chem 289:30889-30899 (2014)

PubMed id: 25217636

Crystal structure of the Bacillus subtilis phosphodiesterase PhoD reveals an iron and calcium-containing active site.

F.Rodriguez, J.Lillington, S.Johnson, C.R.Timmel, S.M.Lea, B.C.Berks.

ABSTRACT

No abstract given.