spacer
spacer

PDBsum entry 2y9h
Go to PDB code: 
protein dna_rna Protein-protein interface(s) links
Hydrolase/RNA PDB id
2y9h

 

 

 

 

Loading ...

 
JSmol PyMol  
Contents
Protein chains
213 a.a.
199 a.a.
177 a.a.
DNA/RNA
Waters ×475
PDB id:
2y9h
Name: Hydrolase/RNA
Title: Structure a of crispr endoribonuclease cse3 bound to 19 nt RNA
Structure: Cse3. Chain: a, c, e, g, i, k, m, o. Synonym: tthb192. Engineered: yes. 5'-r( Up Cp Cp Cp Cp Ap Cp Gp Cp Gp Up Gp Up Gp Gp Gp Dgp Ap Up)-3'. Chain: b, d, f, h, j, l, n, p. Engineered: yes. Other_details: RNA with 2'deoxy modification at g 21
Source: Thermus thermophilus. Organism_taxid: 300852. Strain: hb8. Atcc: 27634. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes
Resolution:
2.50Å     R-factor:   0.232     R-free:   0.290
Authors: D.G.Sashital,M.Jinek,J.A.Doudna
Key ref: D.G.Sashital et al. (2011). An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3. Nat Struct Biol, 18, 680-687. PubMed id: 21572442
Date:
14-Feb-11     Release date:   18-May-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q53WG9  (CAS6_THET8) -  CRISPR-associated endoribonuclease Cse3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
Seq:
Struc: 		211 a.a.
213 a.a.
Protein chains
Pfam   ArchSchema ?
Q53WG9  (CAS6_THET8) -  CRISPR-associated endoribonuclease Cse3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
Seq:
Struc: 		211 a.a.
199 a.a.
Protein chain
Pfam   ArchSchema ?
Q53WG9  (CAS6_THET8) -  CRISPR-associated endoribonuclease Cse3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
Seq:
Struc: 		211 a.a.
177 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

DNA/RNA chains
  U-C-C-C-C-A-C-G-C-G-U-G-U-G-G-G-G-A-U 19 bases
  U-C-C-C-C-A-C-G-C-G-U-G-U-G-G-G-G-A-U 19 bases
  U-C-C-C-C-A-C-G-C-G-U-G-U-G-G-G-G-A-U 19 bases
  U-C-C-C-C-A-C-G-C-G-U-G-U-G-G-G-G-A-U 19 bases
  U-C-C-C-C-A-C-G-C-G-U-G-U-G-G-G-G-A-U 19 bases
  U-C-C-C-C-A-C-G-C-G-U-G-U-G-G-G-G-A-U 19 bases
  U-C-C-C-C-A-C-G-C-G-U-G-U-G-G-G-G-A 18 bases
  U-C-C-C-C-A-C-G-C-G-U-G-U-G-G-G-G-A 18 bases

 Enzyme reactions 
   Enzyme class: Chains A, C, E, G, I, K, M, O: E.C.3.1.-.-
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

 

 
Nat Struct Biol 18:680-687 (2011)
PubMed id: 21572442  
 
 
An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3.
D.G.Sashital, M.Jinek, J.A.Doudna.
 
  ABSTRACT  
 
No abstract given.

 

 

spacer
spacer