PDBsum entry 2y1k

Hydrolase

PDB id: 2y1k

Contents

Protein chain

526 a.a.

Ligands

NAG-FUC

SO4 ×5

GOL

NAG ×7

FUC ×2

Metals

_NA

_CL

Waters ×229

PDB id:

2y1k

Name:

Hydrolase

Title:

Structure of human butyrylcholinesterase inhibited by cbdp (12h soak): phosphoserine adduct

Structure:

Cholinesterase. Chain: a. Fragment: residues 29-557. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: cho-k1.

Resolution:

2.50Å R-factor: 0.185 R-free: 0.247

Authors:

E.Carletti,J.P.Colletier,F.Nachon,M.Weik

Key ref:

E.Carletti et al. (2011). Reaction of cresyl saligenin phosphate, the organophosphorus agent implicated in aerotoxic syndrome, with human cholinesterases: mechanistic studies employing kinetics, mass spectrometry, and X-ray structure analysis. Chem Res Toxicol, 24, 797-808. PubMed id: 21438623

Date:

08-Dec-10 Release date: 29-Jun-11

Protein chain

P06276  (CHLE_HUMAN) -  Cholinesterase from Homo sapiens

Seq: 602 a.a.

Struc: 526 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.1.8 - cholinesterase.
• Reaction: an acylcholine + H2O = a carboxylate + choline + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Chem Res Toxicol 24:797-808 (2011)

PubMed id: 21438623

Reaction of cresyl saligenin phosphate, the organophosphorus agent implicated in aerotoxic syndrome, with human cholinesterases: mechanistic studies employing kinetics, mass spectrometry, and X-ray structure analysis.

E.Carletti, L.M.Schopfer, J.P.Colletier, M.T.Froment, F.Nachon, M.Weik, O.Lockridge, P.Masson.

ABSTRACT

No abstract given.