PDBsum entry 2xr9

Hydrolase

PDB id: 2xr9

Contents

Protein chain

783 a.a. *

Ligands

NAG-NAG-BMA

SCN ×4

PO4

Metals

IOD ×28

_ZN ×2

_CA

Waters ×332

* Residue conservation analysis

PDB id:

2xr9

Name:

Hydrolase

Title:

Crystal structure of autotaxin (enpp2)

Structure:

Ectonucleotide pyrophosphatase/phosphodiesterase family member 2. Chain: a. Synonym: autotaxin enpp2, extracellular lysophospholipase d, lysopld. Engineered: yes. Mutation: yes

Source:

Rattus norvegicus. Norway rat. Organism_taxid: 10116. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_variant: flipin. Expression_system_cell_line: hek293

Resolution:

2.05Å R-factor: 0.175 R-free: 0.221

Authors:

S.Kamtekar,J.Hausmann,J.E.Day,E.Christodoulou,A.Perrakis

Key ref:

J.Hausmann et al. (2011). Structural basis of substrate discrimination and integrin binding by autotaxin. Nat Struct Biol, 18, 198-204. PubMed id: 21240271

Date:

13-Sep-10 Release date: 19-Jan-11

Protein chain

Q64610  (ENPP2_RAT) -  Autotaxin from Rattus norvegicus

Seq: 887 a.a.

Struc: 783 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: E.C.3.1.4.39 - alkylglycerophosphoethanolamine phosphodiesterase.
• Reaction: a 1-O-alkyl-sn-glycero-3-phosphoethanolamine + H2O = a 1-O-alkyl- sn-glycero-3-phosphate + ethanolamine + H⁺

1-O-alkyl-sn-glycero-3-phosphoethanolamine + H2O = 1-O-alkyl- sn-glycero-3-phosphate + ethanolamine (Bound ligand (Het Group name = SCN) matches with 40.00% similarity) + H(+)

• Enzyme class 2: E.C.3.1.4.4 - phospholipase D.
• Reaction: a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero- 3-phosphate + choline + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Nat Struct Biol 18:198-204 (2011)

PubMed id: 21240271

Structural basis of substrate discrimination and integrin binding by autotaxin.

J.Hausmann, S.Kamtekar, E.Christodoulou, J.E.Day, T.Wu, Z.Fulkerson, H.M.Albers, L.A.van Meeteren, A.J.Houben, L.van Zeijl, S.Jansen, M.Andries, T.Hall, L.E.Pegg, T.E.Benson, M.Kasiem, K.Harlos, C.W.Kooi, S.S.Smyth, H.Ovaa, M.Bollen, A.J.Morris, W.H.Moolenaar, A.Perrakis.

ABSTRACT

No abstract given.