PDBsum entry 2xpy

Hydrolase

PDB id

2xpy

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Contents

			Protein chain

					628 a.a. *

			Ligands

					GOL


					GSH

			Metals

					_ZN

			Waters ×114

* Residue conservation analysis

PDB id:

2xpy

Links

Name:

Hydrolase

Title:

Structure of native leukotriene a4 hydrolase from saccharomyces cerevisiae

Structure:

Leukotriene a-4 hydrolase. Chain: a. Fragment: residues 40-671. Synonym: lta-4 hydrolase. Engineered: yes. Other_details: glutathione attached at cys147

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: ril.

Resolution:

2.73Å

R-factor:

0.178

R-free:

0.240

Authors:

C.Helgstrand,M.Hasan,H.Usyal,J.Z.Haeggstrom,M.M.G.M.Thunnissen

Key ref:

C.Helgstrand et al. (2011). A leukotriene A4 hydrolase-related aminopeptidase from yeast undergoes induced fit upon inhibitor binding. J Mol Biol, 406, 120-134. PubMed id: 21146536

Date:

31-Aug-10

Release date:

29-Dec-10

PROCHECK

	Headers

	References

Protein chain

Q10740 (LKHA4_YEAST) - Leucine aminopeptidase 2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:

Seq: Struc:					671 a.a. 628 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class 2:

E.C.3.3.2.10 - soluble epoxide hydrolase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

an epoxide + H2O = an ethanediol

epoxide	+	H2O	=	ethanediol

Enzyme class 3:

E.C.3.4.11.- - ?????

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

	J Mol Biol 406:120-134 (2011)
PubMed id: 21146536

A leukotriene A4 hydrolase-related aminopeptidase from yeast undergoes induced fit upon inhibitor binding.
C.Helgstrand, M.Hasan, H.Uysal, J.Z.Haeggström, M.M.Thunnissen.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21508329

G.Kochan, T.Krojer, D.Harvey, R.Fischer, L.Chen, M.Vollmar, F.von Delft, K.L.Kavanagh, M.A.Brown, P.Bowness, P.Wordsworth, B.M.Kessler, and U.Oppermann (2011).
Crystal structures of the endoplasmic reticulum aminopeptidase-1 (ERAP1) reveal the molecular basis for N-terminal peptide trimming.

Proc Natl Acad Sci U S A, 108, 7745-7750.

PDB code:

3qnf

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.