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PDBsum entry 2xo4
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Oxidoreductase
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PDB id
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2xo4
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Contents |
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* Residue conservation analysis
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PDB id:
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Oxidoreductase
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Title:
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Ribonucleotide reductase y730nh2y modified r1 subunit of e. Coli
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Structure:
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Ribonucleoside-diphosphate reductase 1 subunit alpha. Chain: a, b, c. Fragment: residues 1-761. Synonym: ribonucleoside-diphosphate reductase 1 r1 subunit, ribonucleotide reductase 1, protein b1, ribonucleotide reductase r1 subunit. Engineered: yes. Other_details: site specific incorporation of 3-aminotyrosine at position 730.
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Source:
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Escherichia coli. Organism_taxid: 83333. Strain: k-12. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 562
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Resolution:
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2.50Å
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R-factor:
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0.191
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R-free:
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0.233
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Authors:
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E.C.Minnihan,M.R.Seyedsayamdost,U.Uhlin,J.Stubbe
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Key ref:
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E.C.Minnihan
et al.
(2011).
Kinetics of radical intermediate formation and deoxynucleotide production in 3-aminotyrosine-substituted Escherichia coli ribonucleotide reductases.
J Am Chem Soc,
133,
9430-9440.
PubMed id:
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Date:
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09-Aug-10
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Release date:
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18-Aug-10
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PROCHECK
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Headers
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References
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Enzyme class:
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Chains A, B, C, D, E, F:
E.C.1.17.4.1
- ribonucleoside-diphosphate reductase.
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Reaction:
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a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol
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2'-deoxyribonucleoside diphosphate
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+
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thioredoxin disulfide
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+
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H(2)O
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=
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ribonucleoside diphosphate
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+
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thioredoxin
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Cofactor:
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Fe(3+) or adenosylcob(III)alamin or Mn(2+)
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Fe(3+)
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or
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adenosylcob(III)alamin
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or
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Mn(2+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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J Am Chem Soc
133:9430-9440
(2011)
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PubMed id:
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Kinetics of radical intermediate formation and deoxynucleotide production in 3-aminotyrosine-substituted Escherichia coli ribonucleotide reductases.
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E.C.Minnihan,
M.R.Seyedsayamdost,
U.Uhlin,
J.Stubbe.
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ABSTRACT
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Links
