PDBsum entry 2xhl

Hydrolase

PDB id: 2xhl

Contents

Protein chains

430 a.a. *

410 a.a. *

Metals

_ZN

Waters ×31

* Residue conservation analysis

PDB id:

2xhl

Name:

Hydrolase

Title:

Structure of a functional derivative of clostridium botulinum neurotoxin type b

Structure:

Botulinum neurotoxin b light chain. Chain: a. Fragment: residues 1-437. Synonym: bont/b, bontoxilysin-b. Engineered: yes. Botulinum neurotoxin b heavy chain. Chain: b. Fragment: residues 446-858. Synonym: bont/b, bontoxilysin-b.

Source:

Clostridium botulinum. Organism_taxid: 1491. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

2.80Å R-factor: 0.242 R-free: 0.282

Authors:

G.Masuyer,M.Beard,V.A.Cadd,J.A.Chaddock,K.R.Acharya

Key ref:

G.Masuyer et al. (2011). Structure and activity of a functional derivative of Clostridium botulinum neurotoxin B. J Struct Biol, 174, 52-57. PubMed id: 21078393

Date:

18-Jun-10 Release date: 01-Dec-10

Protein chain

P10844  (BXB_CLOBO) -  Botulinum neurotoxin type B from Clostridium botulinum

Seq: 1291 a.a.

Struc: 430 a.a.*

Protein chain

P10844  (BXB_CLOBO) -  Botulinum neurotoxin type B from Clostridium botulinum

Seq: 1291 a.a.

Struc: 410 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.3.4.24.69 - bontoxilysin.
• Reaction: Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevins, SNAP25 or syntaxin. No detected action on small molecule substrates.
• Cofactor: Zn(2+)

J Struct Biol 174:52-57 (2011)

PubMed id: 21078393

Structure and activity of a functional derivative of Clostridium botulinum neurotoxin B.

G.Masuyer, M.Beard, V.A.Cadd, J.A.Chaddock, K.R.Acharya.

ABSTRACT

No abstract given.