PDBsum entry 2xc8

Viral protein

PDB id

2xc8

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Contents

			Protein chains

					121 a.a.


					12 a.a.

			Waters ×132

PDB id:

2xc8

Links

Name:

Viral protein

Title:

Crystal structure of the gene 22 product of the bacillus subtilis spp1 phage

Structure:

Gene 22 product. Chain: a, b, c. Synonym: bacteriophage spp1 complete nucleotide sequence. Engineered: yes

Source:

Bacillus phage spp1. Organism_taxid: 10724. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.35Å

R-factor:

0.209

R-free:

0.244

Authors:

D.Veesler,S.Blangy,P.Tavares,V.Campanacci,C.Cambillau

Key ref:

D.Veesler et al. (2010). Crystal structure of Bacillus subtilis SPP1 phage gp22 shares fold similarity with a domain of lactococcal phage p2 RBP. Protein Sci, 19, 1439-1443. PubMed id: 20506290

Date:

19-Apr-10

Release date:

09-Jun-10

PROCHECK

	Headers

	References

Protein chains

O48465 (O48465_BPSPP) - Bacteriophage SPP1 complete nucleotide sequence from Bacillus phage SPP1

Seq: Struc:					146 a.a. 121 a.a.*

Protein chain

O48465 (O48465_BPSPP) - Bacteriophage SPP1 complete nucleotide sequence from Bacillus phage SPP1

Seq: Struc:					146 a.a. 12 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

	Protein Sci 19:1439-1443 (2010)
PubMed id: 20506290

Crystal structure of Bacillus subtilis SPP1 phage gp22 shares fold similarity with a domain of lactococcal phage p2 RBP.
D.Veesler, S.Blangy, S.Spinelli, P.Tavares, V.Campanacci, C.Cambillau.

ABSTRACT

SPP1 is a siphophage infecting the gram-positive bacterium Bacillus subtilis. It is constituted by an icosahedric head and a long non-contractile tail formed by gene products (gp) 17-21. A group of 5 small genes (gp 22-24.1) follows in the genome those coding for the main tail components. However, the belonging of the corresponding gp to the tail or to other parts of the phage is not documented. Among these, gp22 lacks sequence identity to any known protein. We report here the gp22 structure solved by X-ray crystallography at 2.35 A resolution. We found that gp22 is a monomer in solution and possesses a significant structural similarity with lactococcal phage p2 ORF 18 N-terminal "shoulder" domain.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20665904

D.Veesler, S.Blangy, J.Lichière, M.Ortiz-Lombarda, P.Tavares, V.Campanacci, and C.Cambillau (2010).
Crystal structure of Bacillus subtilis SPP1 phage gp23.1, a putative chaperone.

Protein Sci, 19, 1812-1816.

PDB codes:

2xf5 2xf6 2xf7

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.