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PDBsum entry 2x9c
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Protein transport
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PDB id
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2x9c
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Protein transport
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Title:
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Crystal structure of a soluble prgi mutant from salmonella typhimurium
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Structure:
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Protein prgi. Chain: a, b. Synonym: prgi. Engineered: yes. Mutation: yes
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Source:
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Salmonella typhimurium. Organism_taxid: 216597. Strain: sl1344. Expressed in: escherichia coli. Expression_system_taxid: 469008.
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Resolution:
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2.45Å
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R-factor:
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0.225
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R-free:
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0.239
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Authors:
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O.Poyraz,H.Schmidt,K.Seidel,F.Delissen,C.Ader,H.Tenenboim,C.Goosmann, B.Laube,A.F.Thuenemann,A.Zychlinsky,M.Baldus,A.Lange,C.Griesinger, M.Kolbe
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Key ref:
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O.Poyraz
et al.
(2010).
Protein refolding is required for assembly of the type three secretion needle.
Nat Struct Biol,
17,
788-792.
PubMed id:
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Date:
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15-Mar-10
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Release date:
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16-Jun-10
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PROCHECK
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Headers
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References
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P41784
(PRGI_SALTY) -
SPI-1 type 3 secretion system needle filament protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
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Seq:
Struc:
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80 a.a.
62 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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*
PDB and UniProt seqs differ
at 2 residue positions (black
crosses)
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Nat Struct Biol
17:788-792
(2010)
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PubMed id:
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Protein refolding is required for assembly of the type three secretion needle.
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O.Poyraz,
H.Schmidt,
K.Seidel,
F.Delissen,
C.Ader,
H.Tenenboim,
C.Goosmann,
B.Laube,
A.F.Thünemann,
A.Zychlinsky,
M.Baldus,
A.Lange,
C.Griesinger,
M.Kolbe.
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ABSTRACT
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Pathogenic Gram-negative bacteria use a type three secretion system (TTSS) to
deliver virulence factors into host cells. Although the order in which proteins
incorporate into the growing TTSS is well described, the underlying assembly
mechanisms are still unclear. Here we show that the TTSS needle protomer refolds
spontaneously to extend the needle from the distal end. We developed a
functional mutant of the needle protomer from Shigella flexneri and Salmonella
typhimurium to study its assembly in vitro. We show that the protomer partially
refolds from alpha-helix into beta-strand conformation to form the TTSS needle.
Reconstitution experiments show that needle growth does not require ATP. Thus,
like the structurally related flagellar systems, the needle elongates by subunit
polymerization at the distal end but requires protomer refolding. Our studies
provide a starting point to understand the molecular assembly mechanisms and the
structure of the TTSS at atomic level.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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L.J.Worrall,
E.Lameignere,
and
N.C.Strynadka
(2011).
Structural overview of the bacterial injectisome.
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Curr Opin Microbiol,
14,
3-8.
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S.Chatterjee,
D.Zhong,
B.A.Nordhues,
K.P.Battaile,
S.Lovell,
and
R.N.De Guzman
(2011).
The crystal structures of the Salmonella type III secretion system tip protein SipD in complex with deoxycholate and chenodeoxycholate.
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Protein Sci,
20,
75-86.
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PDB codes:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
