PDBsum entry 2x7f

Transferase

PDB id: 2x7f

Contents

Protein chains

276 a.a. *

288 a.a. *

236 a.a. *

251 a.a. *

Ligands

824 ×5

Metals

_NA ×5

Waters ×17

* Residue conservation analysis

PDB id:

2x7f

Name:

Transferase

Title:

Crystal structure of the kinase domain of human traf2- and nck- interacting kinase with wee1chk1 inhibitor

Structure:

Traf2 and nck-interacting protein kinase. Chain: a, b, c, d, e. Fragment: kinase domain, residues 1-325. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.

Resolution:

2.80Å R-factor: 0.210 R-free: 0.229

Authors:

M.Vollmar,I.Alfano,B.Shrestha,J.Bray,J.R.C.Muniz,A.Roos, P.Filippakopoulos,N.Burgess-Brown,E.Ugochukwu,O.Gileadi,C.Phillips, P.Mahajan,A.C.W.Pike,O.Fedorov,A.Chaikuad,F.Von Delft,C.Bountra, C.H.Arrowsmith,J.Weigelt,A.Edwards,S.Knapp

Key ref:

M.Vollmar et al. Crystal structure of the kinase domain of human traf2 nck-Interacting kinase with wee1chk1 inhibitor. To be published, .

Date:

26-Feb-10 Release date: 14-Jul-10

Protein chain

Q9UKE5  (TNIK_HUMAN) -  TRAF2 and NCK-interacting protein kinase from Homo sapiens

Seq: 1360 a.a.

Struc: 276 a.a.

Protein chains

Q9UKE5  (TNIK_HUMAN) -  TRAF2 and NCK-interacting protein kinase from Homo sapiens

Seq: 1360 a.a.

Struc: 288 a.a.

Protein chain

Q9UKE5  (TNIK_HUMAN) -  TRAF2 and NCK-interacting protein kinase from Homo sapiens

Seq: 1360 a.a.

Struc: 236 a.a.*

Protein chain

Q9UKE5  (TNIK_HUMAN) -  TRAF2 and NCK-interacting protein kinase from Homo sapiens

Seq: 1360 a.a.

Struc: 251 a.a.

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site