PDBsum entry 2wiz

Hydrolase/DNA

PDB id: 2wiz

Contents

Protein chains

125 a.a. *

DNA/RNA

* Residue conservation analysis

PDB id:

2wiz

Name:

Hydrolase/DNA

Title:

Crystal structures of holliday junction resolvases from archaeoglobus fulgidus bound to DNA substrate

Structure:

Archaeal hjc. Chain: a, b. Fragment: residues 2-136. Engineered: yes. Other_details: n-terminus contains extra residues as a result of cloning procedure. Half-junction. Chain: c. Engineered: yes.

Source:

Archaeoglobus fulgidus dsm 4304. Organism_taxid: 224325. Atcc: 49558. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: plyss. Other_details: german collection of microorganisms (dsm). Synthetic: yes. Synthetic construct.

Resolution:

3.30Å R-factor: 0.208 R-free: 0.283

Authors:

C.Carolis,C.Koehler,C.Sauter,J.Basquin,D.Suck,I.Toeroe

Key ref:

C.Carolis et al. Crystal structures of holliday junction resolvases from archaeoglobus fulgidus bound to DNA substrate. To be published, .

Date:

18-May-09 Release date: 26-May-09

Protein chains

O28314  (HJC_ARCFU) -  Holliday junction resolvase Hjc from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)

Seq: 136 a.a.

Struc: 125 a.a.

DNA/RNA chains

G-G-A-T-C-C-C-T-A-A-G-C-T-C-C-A-T-C-G-A 20 bases

T-C-G-A-T-G-G-A-G-C-T-T-A-G-G-G-A-T-C-C 20 bases

Enzyme reactions

• Enzyme class: E.C.3.1.21.10 - crossover junction endodeoxyribonuclease.