PDBsum entry 2web

Hydrolase

PDB id: 2web

Contents

Protein chain

323 a.a. *

Ligands

MAN ×2

SO4

PP4

Waters ×323

* Residue conservation analysis

PDB id:

2web

Name:

Hydrolase

Title:

Acid proteinase (penicillopepsin) (E.C.3.4.23.20) complex with phosphonate inhibitor: methyl(2s)-[1-(((n-formyl)-l-valyl)amino-2-(2- naphthyl)ethyl)hydroxyphosphinyloxy]-3-phenylpropanoate, sodium salt

Structure:

Penicillopepsin. Chain: a. Ec: 3.4.23.20

Source:

Penicillium janthinellum. Organism_taxid: 5079

Biol. unit:

Dimer (from PQS)

Resolution:

1.50Å R-factor: 0.155 R-free: 0.190

Authors:

J.Ding,M.E.Fraser,M.N.G.James

Key ref:

J.Ding et al. (1998). Macrocyclic inhibitors of penicillopepsin. Ii. X-Ray crystallographic analyses of penicillopepsin complexed with a p3-P1 macrocyclic peptidyl inhibitor and with its two acyclic analogues. J am chem soc, 120, 4610-4621.

Date:

03-Feb-98 Release date: 27-May-98

Supersedes:

1web

Protein chain

P00798  (PEPA1_PENJA) -  Penicillopepsin-1 from Penicillium janthinellum

Seq: 323 a.a.

Struc: 323 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.23.20 - penicillopepsin.
• Reaction: Hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1', but also cleaving 20-Gly-|-Glu-21 in the B chain of insulin. Clots milk, and activates trypsinogen.