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PDBsum entry 2wcg
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Contents |
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* Residue conservation analysis
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PDB id:
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Hydrolase
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Title:
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X-ray structure of acid-beta-glucosidase with n-octyl(cyclic guanidine)-nojirimycin in the active site
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Structure:
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Glucosylceramidase. Chain: a, b. Fragment: residues 40-536. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Expressed in: daucus carota. Expression_system_taxid: 4039
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Resolution:
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2.30Å
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R-factor:
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0.138
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R-free:
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0.194
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Authors:
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B.Brumshtein,M.Aguilar,M.I.Garcia-Moreno,C.O.Mellet,J.M.Garcia- Fernandez,I.Silman,Y.Shaaltiel,D.Aviezer,J.L.Sussman,A.H.Futerman
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Key ref:
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B.Brumshtein
et al.
(2009).
6-Amino-6-deoxy-5,6-di-N-(N'-octyliminomethylidene)nojirimycin: synthesis, biological evaluation, and crystal structure in complex with acid beta-glucosidase.
Chembiochem,
10,
1480-1485.
PubMed id:
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Date:
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12-Mar-09
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Release date:
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24-Nov-09
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PROCHECK
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Headers
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References
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P04062
(GLCM_HUMAN) -
Lysosomal acid glucosylceramidase from Homo sapiens
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Seq:
Struc:
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536 a.a.
493 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class 2:
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E.C.2.4.1.-
- ?????
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Enzyme class 3:
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E.C.3.2.1.-
- ?????
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Enzyme class 4:
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E.C.3.2.1.45
- glucosylceramidase.
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Reaction:
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a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing- 4-enine + D-glucose
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beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine
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+
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H2O
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=
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N-acylsphing- 4-enine
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+
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D-glucose
Bound ligand (Het Group name = )
matches with 91.67% similarity
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Enzyme class 5:
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E.C.3.2.1.46
- galactosylceramidase.
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Reaction:
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a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-galactose
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beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine
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+
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H2O
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=
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N-acylsphing-4-enine
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+
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D-galactose
Bound ligand (Het Group name = )
matches with 91.67% similarity
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Chembiochem
10:1480-1485
(2009)
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PubMed id:
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6-Amino-6-deoxy-5,6-di-N-(N'-octyliminomethylidene)nojirimycin: synthesis, biological evaluation, and crystal structure in complex with acid beta-glucosidase.
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B.Brumshtein,
M.Aguilar-Moncayo,
M.I.García-Moreno,
C.Ortiz Mellet,
J.M.García Fernández,
I.Silman,
Y.Shaaltiel,
D.Aviezer,
J.L.Sussman,
A.H.Futerman.
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ABSTRACT
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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M.Aguilar-Moncayo,
M.I.García-Moreno,
A.Trapero,
M.Egido-Gabás,
A.Llebaria,
J.M.Fernández,
and
C.O.Mellet
(2011).
Bicyclic (galacto)nojirimycin analogues as glycosidase inhibitors: effect of structural modifications in their pharmacological chaperone potential towards β-glucocerebrosidase.
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Org Biomol Chem,
9,
3698-3713.
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E.M.Sánchez-Fernández,
R.Rísquez-Cuadro,
M.Chasseraud,
A.Ahidouch,
C.Ortiz Mellet,
H.Ouadid-Ahidouch,
and
J.M.García Fernández
(2010).
Synthesis of N-, S-, and C-glycoside castanospermine analogues with selective neutral alpha-glucosidase inhibitory activity as antitumour agents.
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Chem Commun (Camb),
46,
5328-5330.
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G.Schitter,
A.J.Steiner,
G.Pototschnig,
E.Scheucher,
M.Thonhofer,
C.A.Tarling,
S.G.Withers,
K.Fantur,
E.Paschke,
D.J.Mahuran,
B.A.Rigat,
M.B.Tropak,
C.Illaszewicz,
R.Saf,
A.E.Stütz,
and
T.M.Wrodnigg
(2010).
Fluorous iminoalditols: a new family of glycosidase inhibitors and pharmacological chaperones.
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Chembiochem,
11,
2026-2033.
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Z.Luan,
K.Higaki,
M.Aguilar-Moncayo,
L.Li,
H.Ninomiya,
E.Nanba,
K.Ohno,
M.I.García-Moreno,
C.Ortiz Mellet,
J.M.García Fernández,
and
Y.Suzuki
(2010).
A Fluorescent sp2-iminosugar with pharmacological chaperone activity for gaucher disease: synthesis and intracellular distribution studies.
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Chembiochem,
11,
2453-2464.
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Z.Luan,
K.Higaki,
M.Aguilar-Moncayo,
H.Ninomiya,
K.Ohno,
M.I.García-Moreno,
C.Ortiz Mellet,
J.M.García Fernández,
and
Y.Suzuki
(2009).
Chaperone activity of bicyclic nojirimycin analogues for Gaucher mutations in comparison with N-(n-nonyl)deoxynojirimycin.
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Chembiochem,
10,
2780-2792.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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