PDBsum entry 2vna

Oxidoreductase

PDB id: 2vna

Contents

Protein chain

349 a.a. *

Ligands

NAP

Waters ×91

* Residue conservation analysis

PDB id:

2vna

Name:

Oxidoreductase

Title:

Structure of human zinc-binding alcohol dehydrogenase 1 (zadh1)

Structure:

Prostaglandin reductase 2. Chain: a. Fragment: catalytic domain, residues 1-349. Synonym: 15-oxoprostaglandin 13-reductase, zinc-binding alcohol dehydrogenase domain-containing protein 1, prg-2, zinc-binding alcohol dehydrogenase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_cell_line: r3-prare2. Other_details: cdna from origene

Resolution:

2.17Å R-factor: 0.192 R-free: 0.226

Authors:

N.Shafqat,K.Kavanagh,A.C.W.Pike,J.R.C.Muniz,E.Pilka,A.K.Roos, S.Picaud,C.Johansson,C.Smee,O.Fedorov,G.Kochan,A.Edwards, C.H.Arrowsmith,J.Weigelt,C.Bountra,F.Von Delft,U.Opperman

Key ref:

N.Shafqat et al. Structure of human zinc-Binding alcohol dehydrogenase 1 (zadh1). To be published, .

Date:

01-Feb-08 Release date: 17-Feb-09

Protein chain

Q8N8N7  (PTGR2_HUMAN) -  Prostaglandin reductase 2 from Homo sapiens

Seq: 351 a.a.

Struc: 349 a.a.

Enzyme reactions

• Enzyme class: E.C.1.3.1.48 - 15-oxoprostaglandin 13-reductase.
• Reaction:
  1. 13,14-dihydro-15-oxo-prostaglandin E2 + NADP⁺ = 15-oxoprostaglandin E2 + NADPH + H⁺
  2. 13,14-dihydro-15-oxo-prostaglandin E2 + NAD⁺ = 15-oxoprostaglandin E2 + NADH + H⁺

13,14-dihydro-15-oxo-prostaglandin E2 + NADP(+) (Bound ligand (Het Group name = NAP) corresponds exactly) = 15-oxoprostaglandin E2 + NADPH + H(+)

13,14-dihydro-15-oxo-prostaglandin E2 + NAD(+) (Bound ligand (Het Group name = NAP) matches with 91.67% similarity) = 15-oxoprostaglandin E2 + NADH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site