PDBsum entry 2uyh

Transferase

PDB id: 2uyh

Contents

Protein chain

327 a.a. *

DNA/RNA

Ligands

SAH

SO4 ×4

Waters ×89

* Residue conservation analysis

PDB id:

2uyh

Name:

Transferase

Title:

Hhai DNA methyltransferase s87q-q237s mutant complex with 13mer gcgc- gmgc oligonucleotide and sah

Structure:

Modification methylase hhai. Chain: a. Synonym: hhai DNA methyltransferase, cytosine-specific methyltransferase hhai, m.Hhai. Engineered: yes. Mutation: yes. 5'-d( Tp Gp Gp Ap Tp G 5Cmp Gp Cp Tp Gp Ap C)-3'. Chain: c. Engineered: yes.

Source:

Haemophilus haemolyticus. Organism_taxid: 726. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes

Resolution:

2.63Å R-factor: 0.199 R-free: 0.258

Authors:

D.Daujotyte,S.Grazulis

Key ref:

D.Daujotyte and s.grazulis Hhai DNA methyltransferase s87q-Q237s mutant complex with 13mer gcgc-Gmgc oligonucleotide and sah. To be published, .

Date:

06-Apr-07 Release date: 27-May-08

Protein chain

P05102  (MTH1_HAEPH) -  Type II methyltransferase M.HhaI from Haemophilus parahaemolyticus

Seq: 327 a.a.

Struc: 327 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

T-G-G-A-T-G-5CM-G-C-T-G-A-C 13 bases

G-T-C-A-G-C-G-C-A-T-C-C 12 bases

Enzyme reactions

• Enzyme class: E.C.2.1.1.37 - Dna (cytosine-5-)-methyltransferase.
• Reaction: a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl- 2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H⁺

2'-deoxycytidine in DNA + S-adenosyl-L-methionine = 5-methyl- 2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+) (Bound ligand (Het Group name = SAH) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site