PDBsum entry 2uyc

Transferase

PDB id: 2uyc

Contents

Protein chain

327 a.a. *

DNA/RNA

Ligands

SAH

SO4 ×6

GOL ×5

Waters ×234

* Residue conservation analysis

PDB id:

2uyc

Name:

Transferase

Title:

Hhai DNA methyltransferase r163n mutant complex with 13mer gcgc-gmgc oligonucleotide and sah

Structure:

Modification methylase hhai. Chain: a. Synonym: cytosine-specific methyltransferase hhai, mhhai, hhai DNA methyltransferase. Engineered: yes. Mutation: yes. 5'-d( Tp Gp Gp Ap Tp Gp 5Cmp Gp Cp Tp Gp Ap C)-3'. Chain: c. Engineered: yes.

Source:

Haemophilus haemolyticus. Organism_taxid: 726. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Organism_taxid: 32630

Resolution:

2.00Å R-factor: 0.200 R-free: 0.227

Authors:

D.Daujotyte,S.Grazulis

Date:

03-Apr-07 Release date: 27-May-08

Protein chain

P05102  (MTH1_HAEPH) -  Type II methyltransferase M.HhaI from Haemophilus parahaemolyticus

Seq: 327 a.a.

Struc: 327 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

T-G-G-A-T-G-5CM-G-C-T-G-A-C 13 bases

G-T-C-A-G-C-G-C-A-T-C-C 12 bases

Enzyme reactions

• Enzyme class: E.C.2.1.1.37 - Dna (cytosine-5-)-methyltransferase.
• Reaction: a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl- 2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H⁺

2'-deoxycytidine in DNA + S-adenosyl-L-methionine = 5-methyl- 2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine (Bound ligand (Het Group name = SAH) corresponds exactly) + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site