PDBsum entry 2sgd

Hydrolase/hydrolase inhibitor

PDB id: 2sgd

Contents

Protein chains

185 a.a. *

51 a.a. *

Ligands

PO4

Metals

__K

Waters ×151

* Residue conservation analysis

PDB id:

2sgd

Name:

Hydrolase/hydrolase inhibitor

Title:

Asp 18 variant of turkey ovomucoid inhibitor third domain complexed with streptomyces griseus proteinase b at ph 10.7

Structure:

Streptogrisin b. Chain: e. Synonym: protease b, sgpb, pronase enzyme b. Ovomucoid. Chain: i. Fragment: third domain. Synonym: asp18-omtky3. Engineered: yes. Mutation: yes

Source:

Streptomyces griseus. Organism_taxid: 1911. Strain: k1. Meleagris gallopavo. Turkey. Organism_taxid: 9103. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Dimer (from PQS)

Resolution:

1.80Å R-factor: 0.196

Authors:

K.Huang,W.Lu,S.Anderson,M.Laskowski Jr.,M.N.G.James

Key ref:

K.Huang et al. To be published, .

Date:

25-Mar-99 Release date: 26-Aug-03

Protein chain

P00777  (PRTB_STRGR) -  Streptogrisin-B from Streptomyces griseus

Seq: 299 a.a.

Struc: 185 a.a.

Protein chain

P68390  (IOVO_MELGA) -  Ovomucoid from Meleagris gallopavo

Seq: 185 a.a.

Struc: 51 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain E: E.C.3.4.21.81 - streptogrisin B.
• Reaction: Hydrolysis of proteins with specificity similar to trypsin.